FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2043287922> ?p ?o ?g. }

• W2043287922 endingPage "485" @default.
• W2043287922 startingPage "477" @default.
• W2043287922 abstract "The novel synthetic retinoid 6-[3-(1-adamantyl)-4-hydroxyphenyl]-2-naphtalene carboxylic acid (AHPN/CD437) has been proven to be a potent inducer of apoptosis in a variety of tumor cell types. However, the mechanism of its action remains to be elucidated. Recent studies suggest that the lysosomal protease cathepsin D, when released from lysosomes to the cytosol, can initiate apoptosis. In this study, we examined whether cathepsin D and free radicals are involved in the CD437-induced apoptosis. Exposure of human leukemia HL-60 cells to CD437 resulted in rapid induction of apoptosis as indicated by caspase activation, phosphatidylserine exposure, mitochondrial alterations and morphological changes. Addition of the antioxidants α-tocopherol acetate effectively inhibited the CD437-induced apoptosis. Measurement of the intracellular free radicals indicated a rise in oxidative stress in CD437-treated cells, which could be attenuated by α-tocopherol acetate. Interestingly, pretreatment of cells with the cathepsin D inhibitor pepstatin A blocked the CD437-induced free radical formation and apoptotic effects, suggesting the involvement of cathepsin D. However, Western blotting revealed no difference in cellular quantity of any forms of cathepsin D between control cells and CD437-treated cells, whereas immunofluorescence analysis of the intracellular distribution of cathepsin D showed release of the enzyme from lysosomes to the cytosol. Labeling of lysosomes with lysosomotropic probes confirmed that CD437 could induce lysosomal leakage. The CD437-induced relocation of cathepsin D could not be prevented by α-tocopherol acetate, suggesting that the lysosomal leakage precedes free radical formation. Furthermore, a retinoic acid nuclear receptor (RAR) antagonist failed to block these effects of CD437, suggesting that the action of CD437 is RAR-independent. Taken together, these data suggest a novel lysosomal pathway for CD437-induced apoptosis, in which lysosomes are the primary target and cathepsin D and free radicals act as death mediators." @default.
• W2043287922 created "2016-06-24" @default.
• W2043287922 creator A5030599421 @default.
• W2043287922 creator A5037878989 @default.
• W2043287922 creator A5051040280 @default.
• W2043287922 creator A5081411983 @default.
• W2043287922 date "2001-05-01" @default.
• W2043287922 modified "2023-10-08" @default.
• W2043287922 title "Evidence of a lysosomal pathway for apoptosis induced by the synthetic retinoid CD437 in human leukemia HL-60 cells" @default.
• W2043287922 cites W14645725 @default.
• W2043287922 cites W1660893922 @default.
• W2043287922 cites W1964198812 @default.
• W2043287922 cites W1981152641 @default.
• W2043287922 cites W1983946174 @default.
• W2043287922 cites W1984254677 @default.
• W2043287922 cites W2008437827 @default.
• W2043287922 cites W2011157209 @default.
• W2043287922 cites W2011257870 @default.
• W2043287922 cites W2013314062 @default.
• W2043287922 cites W2023258120 @default.
• W2043287922 cites W2023317094 @default.
• W2043287922 cites W2023750468 @default.
• W2043287922 cites W2026705036 @default.
• W2043287922 cites W2030772295 @default.
• W2043287922 cites W2049946215 @default.
• W2043287922 cites W2055018120 @default.
• W2043287922 cites W2062445186 @default.
• W2043287922 cites W2069643013 @default.
• W2043287922 cites W2071326040 @default.
• W2043287922 cites W2076296181 @default.
• W2043287922 cites W2077626320 @default.
• W2043287922 cites W2102420815 @default.
• W2043287922 cites W2113999599 @default.
• W2043287922 cites W2123747845 @default.
• W2043287922 cites W2140596567 @default.
• W2043287922 cites W2145518958 @default.
• W2043287922 cites W2152153239 @default.
• W2043287922 cites W2158974589 @default.
• W2043287922 cites W2254093732 @default.
• W2043287922 cites W2316287430 @default.
• W2043287922 cites W2328182378 @default.
• W2043287922 cites W2406258157 @default.
• W2043287922 doi "https://doi.org/10.1038/sj.cdd.4400843" @default.
• W2043287922 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11423908" @default.
• W2043287922 hasPublicationYear "2001" @default.
• W2043287922 type Work @default.
• W2043287922 sameAs 2043287922 @default.
• W2043287922 citedByCount "111" @default.
• W2043287922 countsByYear W20432879222012 @default.
• W2043287922 countsByYear W20432879222013 @default.
• W2043287922 countsByYear W20432879222014 @default.
• W2043287922 countsByYear W20432879222015 @default.
• W2043287922 countsByYear W20432879222016 @default.
• W2043287922 countsByYear W20432879222018 @default.
• W2043287922 countsByYear W20432879222019 @default.
• W2043287922 countsByYear W20432879222020 @default.
• W2043287922 countsByYear W20432879222021 @default.
• W2043287922 countsByYear W20432879222022 @default.
• W2043287922 countsByYear W20432879222023 @default.
• W2043287922 crossrefType "journal-article" @default.
• W2043287922 hasAuthorship W2043287922A5030599421 @default.
• W2043287922 hasAuthorship W2043287922A5037878989 @default.
• W2043287922 hasAuthorship W2043287922A5051040280 @default.
• W2043287922 hasAuthorship W2043287922A5081411983 @default.
• W2043287922 hasBestOaLocation W20432879221 @default.
• W2043287922 hasConcept C104317684 @default.
• W2043287922 hasConcept C167844969 @default.
• W2043287922 hasConcept C173633252 @default.
• W2043287922 hasConcept C181199279 @default.
• W2043287922 hasConcept C185592680 @default.
• W2043287922 hasConcept C190283241 @default.
• W2043287922 hasConcept C2775954498 @default.
• W2043287922 hasConcept C2776350231 @default.
• W2043287922 hasConcept C2776714187 @default.
• W2043287922 hasConcept C2780216420 @default.
• W2043287922 hasConcept C2781121885 @default.
• W2043287922 hasConcept C28021979 @default.
• W2043287922 hasConcept C31573885 @default.
• W2043287922 hasConcept C55493867 @default.
• W2043287922 hasConcept C79879829 @default.
• W2043287922 hasConcept C86803240 @default.
• W2043287922 hasConcept C95444343 @default.
• W2043287922 hasConcept C98424977 @default.
• W2043287922 hasConceptScore W2043287922C104317684 @default.
• W2043287922 hasConceptScore W2043287922C167844969 @default.
• W2043287922 hasConceptScore W2043287922C173633252 @default.
• W2043287922 hasConceptScore W2043287922C181199279 @default.
• W2043287922 hasConceptScore W2043287922C185592680 @default.
• W2043287922 hasConceptScore W2043287922C190283241 @default.
• W2043287922 hasConceptScore W2043287922C2775954498 @default.
• W2043287922 hasConceptScore W2043287922C2776350231 @default.
• W2043287922 hasConceptScore W2043287922C2776714187 @default.
• W2043287922 hasConceptScore W2043287922C2780216420 @default.
• W2043287922 hasConceptScore W2043287922C2781121885 @default.
• W2043287922 hasConceptScore W2043287922C28021979 @default.
• W2043287922 hasConceptScore W2043287922C31573885 @default.
• W2043287922 hasConceptScore W2043287922C55493867 @default.
• W2043287922 hasConceptScore W2043287922C79879829 @default.

• next