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- W2043308932 abstract "In ensemble and single-molecule experiments using the yeast proliferating cell nuclear antigen (PCNA, clamp) and replication factor C (RFC, clamp loader), we have examined the assembly of the RFC·PCNA·DNA complex and its progression to holoenzyme upon addition of polymerase δ (polδ). We obtained data that indicate ( i ) PCNA loading on DNA proceeds through multiple conformational intermediates and is successful after several failed attempts; ( ii ) RFC does not act catalytically on a primed 45-mer templated fork; ( iii ) the RFC·PCNA·DNA complex formed in the presence of ATP is derived from at least two kinetically distinguishable species; ( iv ) these species disassemble through either unloading of RFC·PCNA from DNA or dissociation of PCNA into its component subunits; and ( v ) in the presence of polδ only one species converts to the RFC·PCNA·DNA·pol δ holoenzyme. These findings redefine and deepen our understanding of the clamp-loading process and reveal that it is surprisingly one of trial and error to arrive at a heuristic solution." @default.
- W2043308932 created "2016-06-24" @default.
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- W2043308932 date "2010-11-01" @default.
- W2043308932 modified "2023-10-16" @default.
- W2043308932 title "Stepwise loading of yeast clamp revealed by ensemble and single-molecule studies" @default.
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- W2043308932 doi "https://doi.org/10.1073/pnas.1014139107" @default.
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