Matches in SemOpenAlex for { <https://semopenalex.org/work/W2043326499> ?p ?o ?g. }
Showing items 1 to 68 of
68
with 100 items per page.
- W2043326499 endingPage "300" @default.
- W2043326499 startingPage "300" @default.
- W2043326499 abstract "In vivo fertilized embryos were surgically collected from superovulated dairy cows to evaluate microinjection on embryo development and utilized the polymerase chain reaction technique for selection of transgenic embryos. Seventy-two percent of the embryos with visible pronuclei or nuclei were microinjected with DNA, and the remaining 28% served as uninjected controls. All embryos were cocultured with bovine oviductal epithelial cells. Mean final development scores of embryos within the same initial cell stage at collection were unaffected by microinjection. After 144 h of culture, 45% of the microinjected embryos developed to the morula or blastocyst stage. The transgene was detected in 50, 10, and 9% of demimorulae from embryos microinjected at the 1-, 2-, and 4-cell stages. Frequency of transgene detection was higher in morulae from 1-cell embryos than in morulae from 2- and 4-cell embryos. Use of in vitro coculture, embryo bisection, and polymerase chain reaction technique facilitated selection of bovine embryos that carried the transgene." @default.
- W2043326499 created "2016-06-24" @default.
- W2043326499 creator A5017726447 @default.
- W2043326499 creator A5021886938 @default.
- W2043326499 creator A5036744221 @default.
- W2043326499 creator A5078118608 @default.
- W2043326499 date "1990-01-01" @default.
- W2043326499 modified "2023-10-14" @default.
- W2043326499 title "Culture of early-state caprine embryos using goat oviductal and uterine cell monolayers" @default.
- W2043326499 doi "https://doi.org/10.1016/0093-691x(90)90724-8" @default.
- W2043326499 hasPublicationYear "1990" @default.
- W2043326499 type Work @default.
- W2043326499 sameAs 2043326499 @default.
- W2043326499 citedByCount "9" @default.
- W2043326499 crossrefType "journal-article" @default.
- W2043326499 hasAuthorship W2043326499A5017726447 @default.
- W2043326499 hasAuthorship W2043326499A5021886938 @default.
- W2043326499 hasAuthorship W2043326499A5036744221 @default.
- W2043326499 hasAuthorship W2043326499A5078118608 @default.
- W2043326499 hasConcept C102230213 @default.
- W2043326499 hasConcept C104317684 @default.
- W2043326499 hasConcept C110758877 @default.
- W2043326499 hasConcept C153911025 @default.
- W2043326499 hasConcept C16685009 @default.
- W2043326499 hasConcept C196843134 @default.
- W2043326499 hasConcept C2778177303 @default.
- W2043326499 hasConcept C28748434 @default.
- W2043326499 hasConcept C54355233 @default.
- W2043326499 hasConcept C62525196 @default.
- W2043326499 hasConcept C71924100 @default.
- W2043326499 hasConcept C86803240 @default.
- W2043326499 hasConcept C87073359 @default.
- W2043326499 hasConcept C95444343 @default.
- W2043326499 hasConceptScore W2043326499C102230213 @default.
- W2043326499 hasConceptScore W2043326499C104317684 @default.
- W2043326499 hasConceptScore W2043326499C110758877 @default.
- W2043326499 hasConceptScore W2043326499C153911025 @default.
- W2043326499 hasConceptScore W2043326499C16685009 @default.
- W2043326499 hasConceptScore W2043326499C196843134 @default.
- W2043326499 hasConceptScore W2043326499C2778177303 @default.
- W2043326499 hasConceptScore W2043326499C28748434 @default.
- W2043326499 hasConceptScore W2043326499C54355233 @default.
- W2043326499 hasConceptScore W2043326499C62525196 @default.
- W2043326499 hasConceptScore W2043326499C71924100 @default.
- W2043326499 hasConceptScore W2043326499C86803240 @default.
- W2043326499 hasConceptScore W2043326499C87073359 @default.
- W2043326499 hasConceptScore W2043326499C95444343 @default.
- W2043326499 hasIssue "1" @default.
- W2043326499 hasLocation W20433264991 @default.
- W2043326499 hasOpenAccess W2043326499 @default.
- W2043326499 hasPrimaryLocation W20433264991 @default.
- W2043326499 hasRelatedWork W1964427914 @default.
- W2043326499 hasRelatedWork W2036028667 @default.
- W2043326499 hasRelatedWork W2060146325 @default.
- W2043326499 hasRelatedWork W2088252231 @default.
- W2043326499 hasRelatedWork W2099535287 @default.
- W2043326499 hasRelatedWork W2367018321 @default.
- W2043326499 hasRelatedWork W2378094771 @default.
- W2043326499 hasRelatedWork W2378339968 @default.
- W2043326499 hasRelatedWork W3006388413 @default.
- W2043326499 hasRelatedWork W4308034730 @default.
- W2043326499 hasVolume "33" @default.
- W2043326499 isParatext "false" @default.
- W2043326499 isRetracted "false" @default.
- W2043326499 magId "2043326499" @default.
- W2043326499 workType "article" @default.