FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2043337034> ?p ?o ?g. }

• W2043337034 endingPage "34" @default.
• W2043337034 startingPage "24" @default.
• W2043337034 abstract "Smooth-muscle-cell (SMC) replication and extracellular matrix protein expression are two vital and interrelated processes necessary for normal development of the vasculature. To understand better the nature of this relationship in the developing rat lung, we investigated the relationship between SMC proliferation and the expression of perlecan, a basement membrane (BM) heparan sulfate proteoglycan implicated in the control of SMC growth and differentiation, and tropoelastin (TE), a structural matrix protein not known to influence directly the replicative state of SMCs. Using bromodeoxyuridine (BrdU) incorporation to assess DNA synthesis, we first established the time course of SMC proliferation in the hilar pulmonary artery (PA) from embryonic to adult life. We found a labeling index of > 80% during the embryonic period (embryonic Day 13 [e13] to fetal Day 18 [f18]), a dramatic decline to approximately 40% during the fetal period of development, and a steady decrease in proliferation rates following birth such that, by 30 d of age, a labeling index of < 2% was noted. Using in situ hybridization, we found that although peak expression of both perlecan and TE messenger RNA (mRNA) occurred in the fetal and early postnatal periods following the major decrease in cell replication, TE mRNA expression was clearly observed in the PA as early as embryonic Day 14, whereas perlecan transcripts were virtually undetectable until fetal Day 19. Therefore, to evaluate further the relationship between cell replication and perlecan and/or TE gene expression, we used a combined in situ hybridization/BrdU immunohistochemistry technique and demonstrated that, on an individual cell basis, perlecan message was predominantly expressed by nonreplicating (BrdU-negative) PA, whereas TE mRNA was equally expressed in replicating and nonreplicating PA SMCs. Interestingly, a very similar pattern of replication and relationship to perlecan and TE mRNA expression was noted in airway SMCs and epithelial cells. Thus, in the lung as a whole, maximal expression of both the BM protein perlecan and the interstitial matrix protein TE occurs coordinately and follows the period of maximal SMC proliferation. However, in individual SMCs, perlecan mRNA expression varies inversely with DNA synthesis, whereas TE mRNA expression appears independent of the proliferative state of the cell." @default.
• W2043337034 created "2016-06-24" @default.
• W2043337034 creator A5001433482 @default.
• W2043337034 creator A5014076592 @default.
• W2043337034 creator A5052417193 @default.
• W2043337034 creator A5090857167 @default.
• W2043337034 creator A5091044434 @default.
• W2043337034 date "1999-01-01" @default.
• W2043337034 modified "2023-10-18" @default.
• W2043337034 title "Relationship between Perlecan and Tropoelastin Gene Expression and Cell Replication in the Developing Rat Pulmonary Vasculature" @default.
• W2043337034 cites W101074761 @default.
• W2043337034 cites W1487106955 @default.
• W2043337034 cites W1492952392 @default.
• W2043337034 cites W1498711077 @default.
• W2043337034 cites W1538819561 @default.
• W2043337034 cites W1574987094 @default.
• W2043337034 cites W1664151389 @default.
• W2043337034 cites W1736690088 @default.
• W2043337034 cites W1855999912 @default.
• W2043337034 cites W1909833982 @default.
• W2043337034 cites W1911557575 @default.
• W2043337034 cites W1968465587 @default.
• W2043337034 cites W1974138408 @default.
• W2043337034 cites W1980617596 @default.
• W2043337034 cites W1992037508 @default.
• W2043337034 cites W1996819399 @default.
• W2043337034 cites W1996941257 @default.
• W2043337034 cites W1997208293 @default.
• W2043337034 cites W2003202306 @default.
• W2043337034 cites W2018629974 @default.
• W2043337034 cites W2022142677 @default.
• W2043337034 cites W2028534260 @default.
• W2043337034 cites W2029231027 @default.
• W2043337034 cites W2030244019 @default.
• W2043337034 cites W2031184921 @default.
• W2043337034 cites W2031390084 @default.
• W2043337034 cites W2034771809 @default.
• W2043337034 cites W2036135307 @default.
• W2043337034 cites W2040052703 @default.
• W2043337034 cites W2045965305 @default.
• W2043337034 cites W2047184080 @default.
• W2043337034 cites W2054255117 @default.
• W2043337034 cites W2057842932 @default.
• W2043337034 cites W2060423412 @default.
• W2043337034 cites W2063912731 @default.
• W2043337034 cites W2072890248 @default.
• W2043337034 cites W2078627324 @default.
• W2043337034 cites W2079187682 @default.
• W2043337034 cites W2082332064 @default.
• W2043337034 cites W2086631271 @default.
• W2043337034 cites W2088001213 @default.
• W2043337034 cites W2088631574 @default.
• W2043337034 cites W2092889119 @default.
• W2043337034 cites W2118959961 @default.
• W2043337034 cites W2121057209 @default.
• W2043337034 cites W2121604954 @default.
• W2043337034 cites W2122243862 @default.
• W2043337034 cites W2137570167 @default.
• W2043337034 cites W2142457396 @default.
• W2043337034 cites W2144328981 @default.
• W2043337034 cites W2153373001 @default.
• W2043337034 cites W2161224272 @default.
• W2043337034 cites W2166431337 @default.
• W2043337034 cites W2168402306 @default.
• W2043337034 cites W2188060873 @default.
• W2043337034 cites W2264216940 @default.
• W2043337034 cites W2322832538 @default.
• W2043337034 cites W4211197227 @default.
• W2043337034 doi "https://doi.org/10.1165/ajrcmb.20.1.3321" @default.
• W2043337034 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9870914" @default.
• W2043337034 hasPublicationYear "1999" @default.
• W2043337034 type Work @default.
• W2043337034 sameAs 2043337034 @default.
• W2043337034 citedByCount "23" @default.
• W2043337034 countsByYear W20433370342014 @default.
• W2043337034 countsByYear W20433370342019 @default.
• W2043337034 countsByYear W20433370342022 @default.
• W2043337034 crossrefType "journal-article" @default.
• W2043337034 hasAuthorship W2043337034A5001433482 @default.
• W2043337034 hasAuthorship W2043337034A5014076592 @default.
• W2043337034 hasAuthorship W2043337034A5052417193 @default.
• W2043337034 hasAuthorship W2043337034A5090857167 @default.
• W2043337034 hasAuthorship W2043337034A5091044434 @default.
• W2043337034 hasConcept C104317684 @default.
• W2043337034 hasConcept C145103041 @default.
• W2043337034 hasConcept C150194340 @default.
• W2043337034 hasConcept C153911025 @default.
• W2043337034 hasConcept C172680121 @default.
• W2043337034 hasConcept C189165786 @default.
• W2043337034 hasConcept C22731427 @default.
• W2043337034 hasConcept C2776193487 @default.
• W2043337034 hasConcept C2778260397 @default.
• W2043337034 hasConcept C2779234561 @default.
• W2043337034 hasConcept C2779335624 @default.
• W2043337034 hasConcept C2779869378 @default.
• W2043337034 hasConcept C2780299668 @default.
• W2043337034 hasConcept C54355233 @default.
• W2043337034 hasConcept C62112901 @default.

• next