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• W2043353204 abstract "Stem-like cells have been isolated in tumors such as breast, lung, colon, prostate and brain. A critical issue in all these tumors, especially in glioblastoma mutliforme (GBM), is to identify and isolate tumor initiating cell population(s) to investigate their role in tumor formation, progression, and recurrence. Understanding tumor initiating cell populations will provide clues to finding effective therapeutic approaches for these tumors. The neurosphere assay (NSA) due to its simplicity and reproducibility has been used as the method of choice for isolation and propagation of many of this tumor cells. This protocol demonstrates the neurosphere culture method to isolate and expand stem-like cells in surgically resected human GBM tumor tissue. The procedures include an initial chemical digestion and mechanical dissociation of tumor tissue, and subsequently plating the resulting single cell suspension in NSA culture. After 7-10 days, primary neurospheres of 150-200 μm in diameter can be observed and are ready for further passaging and expansion." @default.
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• W2043353204 date "2011-10-30" @default.
• W2043353204 modified "2023-10-16" @default.
• W2043353204 title "Isolation and Expansion of Human Glioblastoma Multiforme Tumor Cells Using the Neurosphere Assay" @default.
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• W2043353204 doi "https://doi.org/10.3791/3633" @default.
• W2043353204 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3227195" @default.
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