Matches in SemOpenAlex for { <https://semopenalex.org/work/W2043363350> ?p ?o ?g. }
- W2043363350 endingPage "14873" @default.
- W2043363350 startingPage "14863" @default.
- W2043363350 abstract "Peripheral membrane proteins can be targeted to specific organelles or the plasma membrane by differential recognition of phospholipid headgroups. Although molecular determinants of specificity for several headgroups, including phosphatidylserine and phosphoinositides are well defined, specific recognition of the headgroup of the zwitterionic phosphatidylcholine (PC) is less well understood. In cytosolic proteins the cation-π box provides a suitable receptor for choline recognition and binding through the trimethylammonium moiety. In PC, this moiety might provide a sufficient handle to bind to peripheral proteins via a cation-π cage, where the π systems of two or more aromatic residues are within 4-5 Å of the quaternary amine. We prove this hypothesis by engineering the cation-π box into secreted phosphatidylinositol-specific phospholipase C from Staphylococcus aureus, which lacks specific PC recognition. The N254Y/H258Y variant selectively binds PC-enriched vesicles, and x-ray crystallography reveals N254Y/H258Y binds choline and dibutyroylphosphatidylcholine within the cation-π motif. Such simple PC recognition motifs could be engineered into a wide variety of secondary structures providing a generally applicable method for specific recognition of PC." @default.
- W2043363350 created "2016-06-24" @default.
- W2043363350 creator A5010951257 @default.
- W2043363350 creator A5047196423 @default.
- W2043363350 creator A5074120712 @default.
- W2043363350 creator A5079087907 @default.
- W2043363350 creator A5084942532 @default.
- W2043363350 date "2013-05-01" @default.
- W2043363350 modified "2023-10-14" @default.
- W2043363350 title "The Cation-π Box Is a Specific Phosphatidylcholine Membrane Targeting Motif" @default.
- W2043363350 cites W1539796472 @default.
- W2043363350 cites W16780149 @default.
- W2043363350 cites W1785056813 @default.
- W2043363350 cites W1936207504 @default.
- W2043363350 cites W1973450983 @default.
- W2043363350 cites W1986191025 @default.
- W2043363350 cites W1999583932 @default.
- W2043363350 cites W2001024813 @default.
- W2043363350 cites W2001102262 @default.
- W2043363350 cites W2001641653 @default.
- W2043363350 cites W2004152842 @default.
- W2043363350 cites W2012935454 @default.
- W2043363350 cites W2018065242 @default.
- W2043363350 cites W2019672930 @default.
- W2043363350 cites W2019704247 @default.
- W2043363350 cites W2020994777 @default.
- W2043363350 cites W2022176683 @default.
- W2043363350 cites W2023977480 @default.
- W2043363350 cites W2026593599 @default.
- W2043363350 cites W2026792043 @default.
- W2043363350 cites W2028653672 @default.
- W2043363350 cites W2029252838 @default.
- W2043363350 cites W2029581889 @default.
- W2043363350 cites W2029943266 @default.
- W2043363350 cites W2034391603 @default.
- W2043363350 cites W2034636254 @default.
- W2043363350 cites W2043312574 @default.
- W2043363350 cites W2044406786 @default.
- W2043363350 cites W2047438853 @default.
- W2043363350 cites W2050113854 @default.
- W2043363350 cites W2070153703 @default.
- W2043363350 cites W2110399580 @default.
- W2043363350 cites W2121514271 @default.
- W2043363350 cites W2125872855 @default.
- W2043363350 cites W2139049820 @default.
- W2043363350 cites W2144081223 @default.
- W2043363350 cites W2144998676 @default.
- W2043363350 cites W2147666260 @default.
- W2043363350 cites W2163341755 @default.
- W2043363350 cites W2180229411 @default.
- W2043363350 doi "https://doi.org/10.1074/jbc.m113.466532" @default.
- W2043363350 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3663509" @default.
- W2043363350 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23576432" @default.
- W2043363350 hasPublicationYear "2013" @default.
- W2043363350 type Work @default.
- W2043363350 sameAs 2043363350 @default.
- W2043363350 citedByCount "37" @default.
- W2043363350 countsByYear W20433633502013 @default.
- W2043363350 countsByYear W20433633502014 @default.
- W2043363350 countsByYear W20433633502015 @default.
- W2043363350 countsByYear W20433633502016 @default.
- W2043363350 countsByYear W20433633502017 @default.
- W2043363350 countsByYear W20433633502018 @default.
- W2043363350 countsByYear W20433633502019 @default.
- W2043363350 countsByYear W20433633502020 @default.
- W2043363350 countsByYear W20433633502021 @default.
- W2043363350 countsByYear W20433633502022 @default.
- W2043363350 countsByYear W20433633502023 @default.
- W2043363350 crossrefType "journal-article" @default.
- W2043363350 hasAuthorship W2043363350A5010951257 @default.
- W2043363350 hasAuthorship W2043363350A5047196423 @default.
- W2043363350 hasAuthorship W2043363350A5074120712 @default.
- W2043363350 hasAuthorship W2043363350A5079087907 @default.
- W2043363350 hasAuthorship W2043363350A5084942532 @default.
- W2043363350 hasBestOaLocation W20433633501 @default.
- W2043363350 hasConcept C130316041 @default.
- W2043363350 hasConcept C132677234 @default.
- W2043363350 hasConcept C170493617 @default.
- W2043363350 hasConcept C178790620 @default.
- W2043363350 hasConcept C179303850 @default.
- W2043363350 hasConcept C181199279 @default.
- W2043363350 hasConcept C185592680 @default.
- W2043363350 hasConcept C2776330855 @default.
- W2043363350 hasConcept C2776568683 @default.
- W2043363350 hasConcept C2778597717 @default.
- W2043363350 hasConcept C2778918659 @default.
- W2043363350 hasConcept C2780610907 @default.
- W2043363350 hasConcept C32909587 @default.
- W2043363350 hasConcept C41625074 @default.
- W2043363350 hasConcept C55493867 @default.
- W2043363350 hasConcept C62478195 @default.
- W2043363350 hasConcept C71240020 @default.
- W2043363350 hasConcept C98539663 @default.
- W2043363350 hasConceptScore W2043363350C130316041 @default.
- W2043363350 hasConceptScore W2043363350C132677234 @default.
- W2043363350 hasConceptScore W2043363350C170493617 @default.
- W2043363350 hasConceptScore W2043363350C178790620 @default.
- W2043363350 hasConceptScore W2043363350C179303850 @default.