FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2043442015> ?p ?o ?g. }

• W2043442015 abstract "Proceedings: AACR 101st Annual Meeting 2010‐‐ Apr 17‐21, 2010; Washington, DCEndothelial, fibroblast cells, and extracellular matrix play a major role in progression, growth and spread of cancers. The extent to which each plays a role in metabolic adaptations in cancer metabolism is unknown. Because of the unique signature of metabolites in endothelial and fibroblasts when compared with epithelial cancer cell metabolism, metabolic profiling is an ideal approach to study induced metabolic changes in cancer cells during cocultures with fibroblasts and endothelia cells. Additionally, interaction of extracellular matrix with cancer cells has been shown to play a key role in cancer invasion and dissemination. In current work, we study the role of microenvironment (various cell types and extracellular matrix proteins) on alterations and adaptations of cancer metabolism. Metabolic profiling of various cancer cells (lung, prostate, breast, and ovarian) in coculture with fibroblasts and endothelial cells was done using metabolic flux analysis in various extracellular matrix environments (collagen coating, collagen gel, and matrigel). Metabolic flux analysis (MFA) refers to a methodology whereby intracellular fluxes (i.e. conversion rates of metabolites through individual reactions) are calculated using a stoichiometric model for the major intracellular reactions and applying mass balances around intracellular metabolites. MFA is a powerful approach for understanding and comparing different metabolic states, and offer insights into the functional capabilities of a metabolic network. The power of this approach is that it takes into account a large set of measurements as well as the complex inter-dependence among the various pathways due to the sharing of a common pool of co-factors such as NADH, NADPH, thus providing a more complete and integrated picture of the metabolic state than isolated measurements relevant to a few pathways. Our findings using quantification of 82 metabolites pertaining to central carbon and nitrogen metabolism provided clues as to which pathways relevant to glycolysis, the pentose phosphate pathway, the TCA and urea cycles, lipid cycle, and amino acid and nucleotide metabolisms, may be altered by endothelial and fibroblasts in cancer cells, resulting in identification of dominant factors which regulate cancer cell metabolism. During extracellular matrix cultures, amino acid supplementation, irrespective of extracellular matrix type, induced dramatic changes. Amino acid supplementation also significantly increased the TCA cycle flux at the level of citrate synthase and oxidized 96% to 97% of the fatty acid-derived acetyl-CoA to CO2. The information we gain elucidates the critical role of microenvironment and nutritional supplementation in cancer metabolism and allows development of therapeutics for modulating the communication between cancer and its microenvironment to prevent cancer invasion and dissemination.Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 45." @default.
• W2043442015 created "2016-06-24" @default.
• W2043442015 creator A5004078749 @default.
• W2043442015 creator A5014640300 @default.
• W2043442015 creator A5024250418 @default.
• W2043442015 creator A5024961051 @default.
• W2043442015 creator A5038715165 @default.
• W2043442015 creator A5046570931 @default.
• W2043442015 date "2010-04-15" @default.
• W2043442015 modified "2023-09-25" @default.
• W2043442015 title "Abstract 45: Role of microenvironment on alterations in cancer metabolism using metabolic profiling" @default.
• W2043442015 doi "https://doi.org/10.1158/1538-7445.am10-45" @default.
• W2043442015 hasPublicationYear "2010" @default.
• W2043442015 type Work @default.
• W2043442015 sameAs 2043442015 @default.
• W2043442015 citedByCount "0" @default.
• W2043442015 crossrefType "proceedings-article" @default.
• W2043442015 hasAuthorship W2043442015A5004078749 @default.
• W2043442015 hasAuthorship W2043442015A5014640300 @default.
• W2043442015 hasAuthorship W2043442015A5024250418 @default.
• W2043442015 hasAuthorship W2043442015A5024961051 @default.
• W2043442015 hasAuthorship W2043442015A5038715165 @default.
• W2043442015 hasAuthorship W2043442015A5046570931 @default.
• W2043442015 hasConcept C121608353 @default.
• W2043442015 hasConcept C1491633281 @default.
• W2043442015 hasConcept C185592680 @default.
• W2043442015 hasConcept C189165786 @default.
• W2043442015 hasConcept C192989942 @default.
• W2043442015 hasConcept C2776107976 @default.
• W2043442015 hasConcept C2778608917 @default.
• W2043442015 hasConcept C28406088 @default.
• W2043442015 hasConcept C2909706936 @default.
• W2043442015 hasConcept C502942594 @default.
• W2043442015 hasConcept C54355233 @default.
• W2043442015 hasConcept C55493867 @default.
• W2043442015 hasConcept C62231903 @default.
• W2043442015 hasConcept C79879829 @default.
• W2043442015 hasConcept C86803240 @default.
• W2043442015 hasConcept C95444343 @default.
• W2043442015 hasConcept C96232424 @default.
• W2043442015 hasConceptScore W2043442015C121608353 @default.
• W2043442015 hasConceptScore W2043442015C1491633281 @default.
• W2043442015 hasConceptScore W2043442015C185592680 @default.
• W2043442015 hasConceptScore W2043442015C189165786 @default.
• W2043442015 hasConceptScore W2043442015C192989942 @default.
• W2043442015 hasConceptScore W2043442015C2776107976 @default.
• W2043442015 hasConceptScore W2043442015C2778608917 @default.
• W2043442015 hasConceptScore W2043442015C28406088 @default.
• W2043442015 hasConceptScore W2043442015C2909706936 @default.
• W2043442015 hasConceptScore W2043442015C502942594 @default.
• W2043442015 hasConceptScore W2043442015C54355233 @default.
• W2043442015 hasConceptScore W2043442015C55493867 @default.
• W2043442015 hasConceptScore W2043442015C62231903 @default.
• W2043442015 hasConceptScore W2043442015C79879829 @default.
• W2043442015 hasConceptScore W2043442015C86803240 @default.
• W2043442015 hasConceptScore W2043442015C95444343 @default.
• W2043442015 hasConceptScore W2043442015C96232424 @default.
• W2043442015 hasLocation W20434420151 @default.
• W2043442015 hasOpenAccess W2043442015 @default.
• W2043442015 hasPrimaryLocation W20434420151 @default.
• W2043442015 hasRelatedWork W106917877 @default.
• W2043442015 hasRelatedWork W161777538 @default.
• W2043442015 hasRelatedWork W1986733939 @default.
• W2043442015 hasRelatedWork W1996677363 @default.
• W2043442015 hasRelatedWork W2055273658 @default.
• W2043442015 hasRelatedWork W2124716251 @default.
• W2043442015 hasRelatedWork W2274058631 @default.
• W2043442015 hasRelatedWork W2323526544 @default.
• W2043442015 hasRelatedWork W2333727057 @default.
• W2043442015 hasRelatedWork W2492254789 @default.
• W2043442015 hasRelatedWork W2530608816 @default.
• W2043442015 hasRelatedWork W2763966161 @default.
• W2043442015 hasRelatedWork W2777685881 @default.
• W2043442015 hasRelatedWork W2790419205 @default.
• W2043442015 hasRelatedWork W2886826878 @default.
• W2043442015 hasRelatedWork W3005397493 @default.
• W2043442015 hasRelatedWork W3005735687 @default.
• W2043442015 hasRelatedWork W3005918052 @default.
• W2043442015 hasRelatedWork W3106577951 @default.
• W2043442015 hasRelatedWork W3158660301 @default.
• W2043442015 isParatext "false" @default.
• W2043442015 isRetracted "false" @default.
• W2043442015 magId "2043442015" @default.
• W2043442015 workType "article" @default.