FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2043451019> ?p ?o ?g. }

• W2043451019 endingPage "435" @default.
• W2043451019 startingPage "423" @default.
• W2043451019 abstract "The mammalian endoglycosidase heparanase (Hpa1) is primarily responsible for cleaving heparan sulphate proteoglycans (HSPGs) present on the basement membrane of cells and its potential for remodelling the extracellular matrix (ECM) could be important in embryonic development and tumour metastasis. Elevated expression of this enzyme has been implicated in various pathological processes including tumour cell proliferation, metastasis, inflammation and angiogenesis. The enzyme therefore represents a potential therapeutic target. Hpa1 protein is initially synthesized as an inactive 65 kDa proenzyme that is then believed to be subsequently activated by proteolytic cleavage to generate an active heterodimer of 8 and 50 kDa polypeptides. By analysis of a series of Hpa1 deletion proteins we confirm that the 8 kDa subunit is essential for enzyme activity. We present here for the first time an insect cell expression system used for the generation of large amounts of recombinant protein of high specific activity. Individual subunits were cloned into baculoviral secretory vectors and co-expressed in insect cells. Active secreted heterodimer protein was recovered from the medium and isolated by a one-step heparin-Sepharose chromatography procedure to give protein of >90% purity. The recombinant enzyme behaved similarly to the native protein with respect to the size of HS fragments liberated on digestion, substrate cleavage specificity and its preference for acidic pH. A significant amount of activity, however, was also detectable at physiological pH values, as measured both by an in vitro assay and by in vivo degradation of cell-bound heparan sulphate." @default.
• W2043451019 created "2016-06-24" @default.
• W2043451019 creator A5003625639 @default.
• W2043451019 creator A5006311071 @default.
• W2043451019 creator A5009840818 @default.
• W2043451019 creator A5019706003 @default.
• W2043451019 creator A5020117339 @default.
• W2043451019 creator A5024759357 @default.
• W2043451019 creator A5031876902 @default.
• W2043451019 creator A5039864212 @default.
• W2043451019 creator A5044189301 @default.
• W2043451019 creator A5050906207 @default.
• W2043451019 creator A5057816995 @default.
• W2043451019 creator A5062813234 @default.
• W2043451019 creator A5074439039 @default.
• W2043451019 creator A5077642524 @default.
• W2043451019 creator A5078514469 @default.
• W2043451019 creator A5086751502 @default.
• W2043451019 creator A5086778688 @default.
• W2043451019 creator A5086791770 @default.
• W2043451019 creator A5087947375 @default.
• W2043451019 creator A5088772122 @default.
• W2043451019 date "2003-07-15" @default.
• W2043451019 modified "2023-09-27" @default.
• W2043451019 title "Biochemical characterization of the active heterodimer form of human heparanase (Hpa1) protein expressed in insect cells" @default.
• W2043451019 cites W1491748226 @default.
• W2043451019 cites W1519001384 @default.
• W2043451019 cites W1526312778 @default.
• W2043451019 cites W1621137138 @default.
• W2043451019 cites W1796214313 @default.
• W2043451019 cites W1869562643 @default.
• W2043451019 cites W1911803680 @default.
• W2043451019 cites W1934825002 @default.
• W2043451019 cites W1948560807 @default.
• W2043451019 cites W1959298931 @default.
• W2043451019 cites W1967582430 @default.
• W2043451019 cites W1977195767 @default.
• W2043451019 cites W1989676517 @default.
• W2043451019 cites W2003500892 @default.
• W2043451019 cites W2009294883 @default.
• W2043451019 cites W2014678714 @default.
• W2043451019 cites W2027789202 @default.
• W2043451019 cites W2031918451 @default.
• W2043451019 cites W2033341544 @default.
• W2043451019 cites W2037813339 @default.
• W2043451019 cites W2041402726 @default.
• W2043451019 cites W2046723510 @default.
• W2043451019 cites W2047502592 @default.
• W2043451019 cites W2059614240 @default.
• W2043451019 cites W2061051242 @default.
• W2043451019 cites W2061577022 @default.
• W2043451019 cites W2063212907 @default.
• W2043451019 cites W2064040661 @default.
• W2043451019 cites W2071878455 @default.
• W2043451019 cites W2072160034 @default.
• W2043451019 cites W2076097930 @default.
• W2043451019 cites W2082640248 @default.
• W2043451019 cites W2093454357 @default.
• W2043451019 cites W2105301755 @default.
• W2043451019 cites W2107489741 @default.
• W2043451019 cites W2114832246 @default.
• W2043451019 cites W2117548386 @default.
• W2043451019 cites W2123838476 @default.
• W2043451019 cites W2132001104 @default.
• W2043451019 cites W2132670378 @default.
• W2043451019 cites W2136464722 @default.
• W2043451019 cites W2146344567 @default.
• W2043451019 cites W2152433281 @default.
• W2043451019 cites W2159154148 @default.
• W2043451019 cites W2165132516 @default.
• W2043451019 cites W2165897964 @default.
• W2043451019 cites W2169829977 @default.
• W2043451019 cites W2171696900 @default.
• W2043451019 cites W2314559944 @default.
• W2043451019 cites W4251144546 @default.
• W2043451019 doi "https://doi.org/10.1042/bj20030318" @default.
• W2043451019 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1223510" @default.
• W2043451019 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12713442" @default.
• W2043451019 hasPublicationYear "2003" @default.
• W2043451019 type Work @default.
• W2043451019 sameAs 2043451019 @default.
• W2043451019 citedByCount "108" @default.
• W2043451019 countsByYear W20434510192012 @default.
• W2043451019 countsByYear W20434510192013 @default.
• W2043451019 countsByYear W20434510192014 @default.
• W2043451019 countsByYear W20434510192015 @default.
• W2043451019 countsByYear W20434510192016 @default.
• W2043451019 countsByYear W20434510192017 @default.
• W2043451019 countsByYear W20434510192018 @default.
• W2043451019 countsByYear W20434510192019 @default.
• W2043451019 countsByYear W20434510192020 @default.
• W2043451019 countsByYear W20434510192021 @default.
• W2043451019 countsByYear W20434510192022 @default.
• W2043451019 countsByYear W20434510192023 @default.
• W2043451019 crossrefType "journal-article" @default.
• W2043451019 hasAuthorship W2043451019A5003625639 @default.
• W2043451019 hasAuthorship W2043451019A5006311071 @default.
• W2043451019 hasAuthorship W2043451019A5009840818 @default.

• next