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• W2043474816 abstract "We report the first image of an intact, frozen hydrated eukaryotic cell using x-ray diffraction microscopy, or coherent x-ray diffraction imaging. By plunge freezing the specimen in liquid ethane and maintaining it below -170 degrees C, artifacts due to dehydration, ice crystallization, and radiation damage are greatly reduced. In this example, coherent diffraction data using 520 eV x rays were recorded and reconstructed to reveal a budding yeast cell at a resolution better than 25 nm. This demonstration represents an important step towards high resolution imaging of cells in their natural, hydrated state, without limitations imposed by x-ray optics." @default.
• W2043474816 created "2016-06-24" @default.
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• W2043474816 date "2009-11-05" @default.
• W2043474816 modified "2023-10-16" @default.
• W2043474816 title "Soft X-Ray Diffraction Microscopy of a Frozen Hydrated Yeast Cell" @default.
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• W2043474816 doi "https://doi.org/10.1103/physrevlett.103.198101" @default.
• W2043474816 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2866741" @default.
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• W2043474816 hasPublicationYear "2009" @default.
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