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- W2043485281 abstract "A highly sensitive reverse transcriptase-polymerase chain reaction (RT-PCR) assay and a flow cytometric assay were used to examine ACHN cells for the expression of P-glycoprotein. The expression of P-glycoprotein was detected at the RNA and protein levels in ACHN cells by RT-PCR and flow cytometry, respectively. However, it was below the limit of detection by immunoblotting. The intracellular accumulation of adriamycin in ACHN cells was enhanced by verapamil, cyclosporin A and medroxyprogesterone acetate. Therefore, this study has demonstrated that low-level expression of P-glycoprotein detectable only by RT-PCR and flow cytometry plays a significant role in reducing the intracellular concentration of antitumor agents and thus contributes to the multidrug-resistant phenotype of ACHN cells." @default.
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- W2043485281 date "1996-05-01" @default.
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- W2043485281 title "Detection of Low-level Expression of P-Glycoprotein in ACHN Renal Adenocarcinoma Cells" @default.
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- W2043485281 doi "https://doi.org/10.1111/j.1349-7006.1996.tb00248.x" @default.
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