FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2043615791> ?p ?o ?g. }

• W2043615791 endingPage "4478" @default.
• W2043615791 startingPage "4473" @default.
• W2043615791 abstract "We have previously isolated a cDNA encoding a human uracil-DNA glycosylase which is closely related to the bacterial and yeast enzymes. In vitro expression of this cDNA produced a protein with an apparent molecular weight of 34 K in agreement with the size predicted from the sequence data. The in vitro expressed protein exhibited uracil-DNA glycosylase activity. The close resemblance between the human and the bacterial enzyme raised the possibility that the human enzyme may be able to complement E. coli ung mutants. In order to test this hypothesis, the human uracil-DNA glycosylase cDNA was established in a bacterial expression vector. Expression of the human enzyme as a LacZα -humUNG fusion protein was then studied in E. coli ung mutants. E. coli cells lacking uracil-DNA glycosylase activity exhibit a weak mutator phenotype and they are permissive for growth of phages with uracil-containing DNA. Here we show that the expression of human uracil-DNA glycosylase in E. coli can restore the wild type phenotype of ung mutants. These results demonstrate that the evolutionary conservation of the uracil-DNA glycosylase structure is also reflected in the conservation of the mechanism for removal of uracil from DNA." @default.
• W2043615791 created "2016-06-24" @default.
• W2043615791 creator A5009412727 @default.
• W2043615791 creator A5025020416 @default.
• W2043615791 creator A5042668202 @default.
• W2043615791 creator A5050686032 @default.
• W2043615791 date "1991-01-01" @default.
• W2043615791 modified "2023-09-25" @default.
• W2043615791 title "Human uracil-DNA glycosylase complementsE.coli ungmutants" @default.
• W2043615791 cites W1377731 @default.
• W2043615791 cites W138005989 @default.
• W2043615791 cites W1479885662 @default.
• W2043615791 cites W1483198897 @default.
• W2043615791 cites W1496246291 @default.
• W2043615791 cites W1516191771 @default.
• W2043615791 cites W1564478425 @default.
• W2043615791 cites W1588506815 @default.
• W2043615791 cites W1588567003 @default.
• W2043615791 cites W1602263916 @default.
• W2043615791 cites W1608297032 @default.
• W2043615791 cites W1967242089 @default.
• W2043615791 cites W1972748983 @default.
• W2043615791 cites W1982227886 @default.
• W2043615791 cites W2000140580 @default.
• W2043615791 cites W2001586358 @default.
• W2043615791 cites W2007639816 @default.
• W2043615791 cites W2011389780 @default.
• W2043615791 cites W2016134181 @default.
• W2043615791 cites W2018289835 @default.
• W2043615791 cites W2021241545 @default.
• W2043615791 cites W2032118018 @default.
• W2043615791 cites W2034622366 @default.
• W2043615791 cites W2035336844 @default.
• W2043615791 cites W2036931150 @default.
• W2043615791 cites W2037978830 @default.
• W2043615791 cites W2053701783 @default.
• W2043615791 cites W2058699163 @default.
• W2043615791 cites W2078444038 @default.
• W2043615791 cites W2086632920 @default.
• W2043615791 cites W2090377242 @default.
• W2043615791 cites W2100837269 @default.
• W2043615791 cites W2116408061 @default.
• W2043615791 cites W2122317093 @default.
• W2043615791 cites W2152845055 @default.
• W2043615791 cites W2345074354 @default.
• W2043615791 cites W68241218 @default.
• W2043615791 doi "https://doi.org/10.1093/nar/19.16.4473" @default.
• W2043615791 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/328636" @default.
• W2043615791 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1886771" @default.
• W2043615791 hasPublicationYear "1991" @default.
• W2043615791 type Work @default.
• W2043615791 sameAs 2043615791 @default.
• W2043615791 citedByCount "33" @default.
• W2043615791 countsByYear W20436157912012 @default.
• W2043615791 countsByYear W20436157912015 @default.
• W2043615791 countsByYear W20436157912019 @default.
• W2043615791 crossrefType "journal-article" @default.
• W2043615791 hasAuthorship W2043615791A5009412727 @default.
• W2043615791 hasAuthorship W2043615791A5025020416 @default.
• W2043615791 hasAuthorship W2043615791A5042668202 @default.
• W2043615791 hasAuthorship W2043615791A5050686032 @default.
• W2043615791 hasBestOaLocation W20436157912 @default.
• W2043615791 hasConcept C104317684 @default.
• W2043615791 hasConcept C134935766 @default.
• W2043615791 hasConcept C153911025 @default.
• W2043615791 hasConcept C187882448 @default.
• W2043615791 hasConcept C192396546 @default.
• W2043615791 hasConcept C2778711568 @default.
• W2043615791 hasConcept C2779554091 @default.
• W2043615791 hasConcept C552990157 @default.
• W2043615791 hasConcept C55493867 @default.
• W2043615791 hasConcept C86803240 @default.
• W2043615791 hasConceptScore W2043615791C104317684 @default.
• W2043615791 hasConceptScore W2043615791C134935766 @default.
• W2043615791 hasConceptScore W2043615791C153911025 @default.
• W2043615791 hasConceptScore W2043615791C187882448 @default.
• W2043615791 hasConceptScore W2043615791C192396546 @default.
• W2043615791 hasConceptScore W2043615791C2778711568 @default.
• W2043615791 hasConceptScore W2043615791C2779554091 @default.
• W2043615791 hasConceptScore W2043615791C552990157 @default.
• W2043615791 hasConceptScore W2043615791C55493867 @default.
• W2043615791 hasConceptScore W2043615791C86803240 @default.
• W2043615791 hasIssue "16" @default.
• W2043615791 hasLocation W20436157911 @default.
• W2043615791 hasLocation W20436157912 @default.
• W2043615791 hasLocation W20436157913 @default.
• W2043615791 hasOpenAccess W2043615791 @default.
• W2043615791 hasPrimaryLocation W20436157911 @default.
• W2043615791 hasRelatedWork W1974890447 @default.
• W2043615791 hasRelatedWork W2026813999 @default.
• W2043615791 hasRelatedWork W2053801105 @default.
• W2043615791 hasRelatedWork W2058636601 @default.
• W2043615791 hasRelatedWork W2116752163 @default.
• W2043615791 hasRelatedWork W2131141230 @default.
• W2043615791 hasRelatedWork W2154129568 @default.
• W2043615791 hasRelatedWork W2277886745 @default.
• W2043615791 hasRelatedWork W2345318811 @default.
• W2043615791 hasRelatedWork W2904639898 @default.

• next