FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2043669500> ?p ?o ?g. }

• W2043669500 endingPage "60" @default.
• W2043669500 startingPage "49" @default.
• W2043669500 abstract "Mammary gland polysomes are difficult to isolate from the lactating rat using methods developed for other species and tissues, most likely due to high calcium-stimulated ribonuclease activity in that tissue. A new method, utilizing ethyleneglycol-bis(β-aminoethylether)-N,N′-tetraacetic acid (EGTA) to bind calcium, yields highly aggregated polysomes from lactating rat mammary gland. Fresh mammary tissue is pulverized under liquid nitrogen. Free and membrane-bound polysomes are isolated by differential centrifugation in solutions containing 100 mK KCl, 100 mM MgCl2, 75 mM EGTA, 500 μg/ml heparin and 50 mM Tris buffer, pH 8.2 at 5°C. Bound polysomes are released from the endoplasmic reticulum using Triton X-100 and deoxycholate. Polysome profiles are obtained on linear sucrose gradients and scanned at 254 nm. The method gives quantitative recovery of homogenate total RNA. To demonstrate that the method can be used to study nutritional effects on mammary gland polysome aggregation, lactating rats were fasted 22–66 h and then refed a stock diet for 71–95 h. Refeeding increased the percentage of polysomes (trimmers or larger) in the bound fraction from 84±1 to 93±1% (P < 0.001) and in the free fraction from 42±2 to 55±3% (P < 0.001)." @default.
• W2043669500 created "2016-06-24" @default.
• W2043669500 creator A5027890860 @default.
• W2043669500 creator A5090548519 @default.
• W2043669500 date "1984-03-01" @default.
• W2043669500 modified "2023-10-14" @default.
• W2043669500 title "A method for isolation of undergraded free and membrane-bound ribosomes from rat lactating mammary gland" @default.
• W2043669500 cites W142318654 @default.
• W2043669500 cites W1486066729 @default.
• W2043669500 cites W1497221394 @default.
• W2043669500 cites W1566969796 @default.
• W2043669500 cites W1591139476 @default.
• W2043669500 cites W1598762083 @default.
• W2043669500 cites W1796140619 @default.
• W2043669500 cites W188926460 @default.
• W2043669500 cites W1968186479 @default.
• W2043669500 cites W1968300236 @default.
• W2043669500 cites W1976658927 @default.
• W2043669500 cites W1979346605 @default.
• W2043669500 cites W2005489013 @default.
• W2043669500 cites W2006803749 @default.
• W2043669500 cites W2009176719 @default.
• W2043669500 cites W2020990440 @default.
• W2043669500 cites W2039946682 @default.
• W2043669500 cites W2043469241 @default.
• W2043669500 cites W2047570451 @default.
• W2043669500 cites W2048502645 @default.
• W2043669500 cites W2053555355 @default.
• W2043669500 cites W2056072167 @default.
• W2043669500 cites W2060581152 @default.
• W2043669500 cites W2060894279 @default.
• W2043669500 cites W2062966606 @default.
• W2043669500 cites W2067927616 @default.
• W2043669500 cites W2075134649 @default.
• W2043669500 cites W2076424193 @default.
• W2043669500 cites W2119282315 @default.
• W2043669500 cites W2154347114 @default.
• W2043669500 cites W267803908 @default.
• W2043669500 cites W77781458 @default.
• W2043669500 cites W2004226154 @default.
• W2043669500 doi "https://doi.org/10.1016/0165-022x(84)90065-4" @default.
• W2043669500 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6427319" @default.
• W2043669500 hasPublicationYear "1984" @default.
• W2043669500 type Work @default.
• W2043669500 sameAs 2043669500 @default.
• W2043669500 citedByCount "2" @default.
• W2043669500 countsByYear W20436695002012 @default.
• W2043669500 crossrefType "journal-article" @default.
• W2043669500 hasAuthorship W2043669500A5027890860 @default.
• W2043669500 hasAuthorship W2043669500A5090548519 @default.
• W2043669500 hasConcept C104317684 @default.
• W2043669500 hasConcept C121608353 @default.
• W2043669500 hasConcept C126322002 @default.
• W2043669500 hasConcept C132946820 @default.
• W2043669500 hasConcept C134018914 @default.
• W2043669500 hasConcept C158617107 @default.
• W2043669500 hasConcept C185592680 @default.
• W2043669500 hasConcept C189976730 @default.
• W2043669500 hasConcept C202751555 @default.
• W2043669500 hasConcept C2777658833 @default.
• W2043669500 hasConcept C2778001805 @default.
• W2043669500 hasConcept C2778976237 @default.
• W2043669500 hasConcept C2781014771 @default.
• W2043669500 hasConcept C519063684 @default.
• W2043669500 hasConcept C530470458 @default.
• W2043669500 hasConcept C55493867 @default.
• W2043669500 hasConcept C65666806 @default.
• W2043669500 hasConcept C67705224 @default.
• W2043669500 hasConcept C71924100 @default.
• W2043669500 hasConcept C7623868 @default.
• W2043669500 hasConcept C86803240 @default.
• W2043669500 hasConcept C88478588 @default.
• W2043669500 hasConceptScore W2043669500C104317684 @default.
• W2043669500 hasConceptScore W2043669500C121608353 @default.
• W2043669500 hasConceptScore W2043669500C126322002 @default.
• W2043669500 hasConceptScore W2043669500C132946820 @default.
• W2043669500 hasConceptScore W2043669500C134018914 @default.
• W2043669500 hasConceptScore W2043669500C158617107 @default.
• W2043669500 hasConceptScore W2043669500C185592680 @default.
• W2043669500 hasConceptScore W2043669500C189976730 @default.
• W2043669500 hasConceptScore W2043669500C202751555 @default.
• W2043669500 hasConceptScore W2043669500C2777658833 @default.
• W2043669500 hasConceptScore W2043669500C2778001805 @default.
• W2043669500 hasConceptScore W2043669500C2778976237 @default.
• W2043669500 hasConceptScore W2043669500C2781014771 @default.
• W2043669500 hasConceptScore W2043669500C519063684 @default.
• W2043669500 hasConceptScore W2043669500C530470458 @default.
• W2043669500 hasConceptScore W2043669500C55493867 @default.
• W2043669500 hasConceptScore W2043669500C65666806 @default.
• W2043669500 hasConceptScore W2043669500C67705224 @default.
• W2043669500 hasConceptScore W2043669500C71924100 @default.
• W2043669500 hasConceptScore W2043669500C7623868 @default.
• W2043669500 hasConceptScore W2043669500C86803240 @default.
• W2043669500 hasConceptScore W2043669500C88478588 @default.
• W2043669500 hasIssue "1" @default.
• W2043669500 hasLocation W20436695001 @default.
• W2043669500 hasLocation W20436695002 @default.
• W2043669500 hasOpenAccess W2043669500 @default.

• next