FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2043698583> ?p ?o ?g. }

• W2043698583 endingPage "893" @default.
• W2043698583 startingPage "890" @default.
• W2043698583 abstract "Although several alternative antivirals are available for the treatment of influenza virus infection, the risk that viruses resistant to these drugs will emerge means it is important to continue the search for new antiviral targets. Hao et al. have recruited a new ally to this cause: by modifying influenza virus so that it can infect the cells of the fruit fly Drosophila, they have been able to use a powerful genome-wide RNA interference (RNAi) screen to identify scores of host genes that the pathogen requires for successful infection. Several host proteins were found that have key functions in the replication of H5N1 and H1N1 influenza A viruses — but not other viruses — in human cells. The same strategy should be applicable to other viruses, as long as at least part of their replication cycle can be supported in Drosophila cells. In this paper, a screening system based on the specific targeting of Drosophila genes with an RNAi library identifies host factors involved in influenza virus replication. All viruses rely on host cell proteins and their associated mechanisms to complete the viral life cycle. Identifying the host molecules that participate in each step of virus replication could provide valuable new targets for antiviral therapy, but this goal may take several decades to achieve with conventional forward genetic screening methods and mammalian cell cultures. Here we describe a novel genome-wide RNA interference (RNAi) screen in Drosophila1 that can be used to identify host genes important for influenza virus replication. After modifying influenza virus to allow infection of Drosophila cells and detection of influenza virus gene expression, we tested an RNAi library against 13,071 genes (90% of the Drosophila genome), identifying over 100 for which suppression in Drosophila cells significantly inhibited or stimulated reporter gene (Renilla luciferase) expression from an influenza-virus-derived vector. The relevance of these findings to influenza virus infection of mammalian cells is illustrated for a subset of the Drosophila genes identified; that is, for three implicated Drosophila genes, the corresponding human homologues ATP6V0D1, COX6A1 and NXF1 are shown to have key functions in the replication of H5N1 and H1N1 influenza A viruses, but not vesicular stomatitis virus or vaccinia virus, in human HEK 293 cells. Thus, we have demonstrated the feasibility of using genome-wide RNAi screens in Drosophila to identify previously unrecognized host proteins that are required for influenza virus replication. This could accelerate the development of new classes of antiviral drugs for chemoprophylaxis and treatment, which are urgently needed given the obstacles to rapid development of an effective vaccine against pandemic influenza and the probable emergence of strains resistant to available drugs." @default.
• W2043698583 created "2016-06-24" @default.
• W2043698583 creator A5001069528 @default.
• W2043698583 creator A5011877382 @default.
• W2043698583 creator A5022522659 @default.
• W2043698583 creator A5026899432 @default.
• W2043698583 creator A5072759658 @default.
• W2043698583 creator A5076363097 @default.
• W2043698583 creator A5085088345 @default.
• W2043698583 creator A5090363279 @default.
• W2043698583 date "2008-07-09" @default.
• W2043698583 modified "2023-10-18" @default.
• W2043698583 title "Drosophila RNAi screen identifies host genes important for influenza virus replication" @default.
• W2043698583 cites W1936689179 @default.
• W2043698583 cites W1946456935 @default.
• W2043698583 cites W1970497184 @default.
• W2043698583 cites W1976670588 @default.
• W2043698583 cites W2001056362 @default.
• W2043698583 cites W2015597206 @default.
• W2043698583 cites W2018481690 @default.
• W2043698583 cites W2044728266 @default.
• W2043698583 cites W2048502224 @default.
• W2043698583 cites W2068799885 @default.
• W2043698583 cites W2083867677 @default.
• W2043698583 cites W2084705887 @default.
• W2043698583 cites W2091080506 @default.
• W2043698583 cites W2101363646 @default.
• W2043698583 cites W2106367530 @default.
• W2043698583 cites W2112790267 @default.
• W2043698583 cites W2119779742 @default.
• W2043698583 cites W2124176030 @default.
• W2043698583 cites W2127707318 @default.
• W2043698583 cites W2128702749 @default.
• W2043698583 cites W2132783730 @default.
• W2043698583 cites W2133106116 @default.
• W2043698583 cites W2133318493 @default.
• W2043698583 cites W2137590150 @default.
• W2043698583 cites W2143341850 @default.
• W2043698583 cites W2143353745 @default.
• W2043698583 cites W2616345050 @default.
• W2043698583 cites W4253422011 @default.
• W2043698583 doi "https://doi.org/10.1038/nature07151" @default.
• W2043698583 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2574945" @default.
• W2043698583 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18615016" @default.
• W2043698583 hasPublicationYear "2008" @default.
• W2043698583 type Work @default.
• W2043698583 sameAs 2043698583 @default.
• W2043698583 citedByCount "393" @default.
• W2043698583 countsByYear W20436985832012 @default.
• W2043698583 countsByYear W20436985832013 @default.
• W2043698583 countsByYear W20436985832014 @default.
• W2043698583 countsByYear W20436985832015 @default.
• W2043698583 countsByYear W20436985832016 @default.
• W2043698583 countsByYear W20436985832017 @default.
• W2043698583 countsByYear W20436985832018 @default.
• W2043698583 countsByYear W20436985832019 @default.
• W2043698583 countsByYear W20436985832020 @default.
• W2043698583 countsByYear W20436985832021 @default.
• W2043698583 countsByYear W20436985832022 @default.
• W2043698583 countsByYear W20436985832023 @default.
• W2043698583 crossrefType "journal-article" @default.
• W2043698583 hasAuthorship W2043698583A5001069528 @default.
• W2043698583 hasAuthorship W2043698583A5011877382 @default.
• W2043698583 hasAuthorship W2043698583A5022522659 @default.
• W2043698583 hasAuthorship W2043698583A5026899432 @default.
• W2043698583 hasAuthorship W2043698583A5072759658 @default.
• W2043698583 hasAuthorship W2043698583A5076363097 @default.
• W2043698583 hasAuthorship W2043698583A5085088345 @default.
• W2043698583 hasAuthorship W2043698583A5090363279 @default.
• W2043698583 hasBestOaLocation W20436985831 @default.
• W2043698583 hasConcept C104317684 @default.
• W2043698583 hasConcept C126831891 @default.
• W2043698583 hasConcept C127716648 @default.
• W2043698583 hasConcept C136961756 @default.
• W2043698583 hasConcept C140704245 @default.
• W2043698583 hasConcept C141231307 @default.
• W2043698583 hasConcept C159047783 @default.
• W2043698583 hasConcept C166703698 @default.
• W2043698583 hasConcept C207177822 @default.
• W2043698583 hasConcept C2522874641 @default.
• W2043698583 hasConcept C2777546802 @default.
• W2043698583 hasConcept C54355233 @default.
• W2043698583 hasConcept C67705224 @default.
• W2043698583 hasConcept C70721500 @default.
• W2043698583 hasConcept C86803240 @default.
• W2043698583 hasConceptScore W2043698583C104317684 @default.
• W2043698583 hasConceptScore W2043698583C126831891 @default.
• W2043698583 hasConceptScore W2043698583C127716648 @default.
• W2043698583 hasConceptScore W2043698583C136961756 @default.
• W2043698583 hasConceptScore W2043698583C140704245 @default.
• W2043698583 hasConceptScore W2043698583C141231307 @default.
• W2043698583 hasConceptScore W2043698583C159047783 @default.
• W2043698583 hasConceptScore W2043698583C166703698 @default.
• W2043698583 hasConceptScore W2043698583C207177822 @default.
• W2043698583 hasConceptScore W2043698583C2522874641 @default.
• W2043698583 hasConceptScore W2043698583C2777546802 @default.
• W2043698583 hasConceptScore W2043698583C54355233 @default.
• W2043698583 hasConceptScore W2043698583C67705224 @default.

• next