FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2043774268> ?p ?o ?g. }

• W2043774268 endingPage "843" @default.
• W2043774268 startingPage "833" @default.
• W2043774268 abstract "Yen1 is a nuclease identified in Saccharomyces cerevisiae that cleaves the Holliday junction (HJ) intermediate formed during homologous recombination. Alternative routes to disjoin HJs are performed by the Mus81/Mms4- and Sgs1/Top3/Rmi1-complexes. Here, we investigate the role of the Yen1 protein in the yeast Kluyveromyces lactis. We demonstrate that both yen1 mus81 and yen1 sgs1 double mutants displayed negative genetic interactions in the presence of DNA-damaging chemicals. To test if these phenotypes required the catalytic activity of Yen1, we introduced point mutations targeting the catalytic site of Yen1, which abolished the nuclease activity in vitro. Remarkably, catalytically inactive Yen1 did not exacerbate the hydroxyurea sensitivity of the sgs1Δ strain, which the yen1Δ allele did. In addition, overexpression of catalytically inactive Yen1 partially rescued the DNA damage sensitivity of both mus81 and sgs1 mutant strains albeit less efficiently than WT Yen1. These results suggest that Yen1 serves both a catalytic and non-catalytic role in its redundant function with Mus81 and Sgs1. Diploids lacking Mus81 had a severe defect in sporulation efficiency and crossover frequency, but diploids lacking both Mus81 and Yen1 showed no further reduction in spore formation. Hence, Yen1 had no evident role in meiosis. However, overexpression of WT Yen1, but not catalytically inactive Yen1 partially rescued the crossover defect in mus81/mus81 mutant diploids. Yen1 is a member of the RAD2/XPG-family of nucleases, but genetic analyses revealed no genetic interaction between yen1 and other family members (rad2, exo1 and rad27). In addition, yen1 mutants had normal nonhomologous end-joining efficiency. We discuss the similarities and differences between K. lactis Yen1 and Yen1/GEN1 from other organisms." @default.
• W2043774268 created "2016-06-24" @default.
• W2043774268 creator A5032296502 @default.
• W2043774268 creator A5034608225 @default.
• W2043774268 date "2012-10-01" @default.
• W2043774268 modified "2023-10-14" @default.
• W2043774268 title "A catalytic and non-catalytic role for the Yen1 nuclease in maintaining genome integrity in Kluyveromyces lactis" @default.
• W2043774268 cites W1877801782 @default.
• W2043774268 cites W1964616851 @default.
• W2043774268 cites W1967491579 @default.
• W2043774268 cites W1967667819 @default.
• W2043774268 cites W1972180308 @default.
• W2043774268 cites W1972852754 @default.
• W2043774268 cites W1976019826 @default.
• W2043774268 cites W1976362728 @default.
• W2043774268 cites W1976708245 @default.
• W2043774268 cites W1990265320 @default.
• W2043774268 cites W1997859335 @default.
• W2043774268 cites W2001005729 @default.
• W2043774268 cites W2001381163 @default.
• W2043774268 cites W2025972514 @default.
• W2043774268 cites W2028138760 @default.
• W2043774268 cites W2034252391 @default.
• W2043774268 cites W2041945142 @default.
• W2043774268 cites W2053577515 @default.
• W2043774268 cites W2054693978 @default.
• W2043774268 cites W2061311583 @default.
• W2043774268 cites W2065761939 @default.
• W2043774268 cites W2066216489 @default.
• W2043774268 cites W2071106544 @default.
• W2043774268 cites W2072707948 @default.
• W2043774268 cites W2078532616 @default.
• W2043774268 cites W2079027867 @default.
• W2043774268 cites W2079185082 @default.
• W2043774268 cites W2082447947 @default.
• W2043774268 cites W2091262522 @default.
• W2043774268 cites W2094046364 @default.
• W2043774268 cites W2095701756 @default.
• W2043774268 cites W2103972950 @default.
• W2043774268 cites W2107856790 @default.
• W2043774268 cites W2108572433 @default.
• W2043774268 cites W2127073167 @default.
• W2043774268 cites W2128638726 @default.
• W2043774268 cites W2131856778 @default.
• W2043774268 cites W2134536765 @default.
• W2043774268 cites W2135657415 @default.
• W2043774268 cites W2141425812 @default.
• W2043774268 cites W2165385395 @default.
• W2043774268 cites W2166911703 @default.
• W2043774268 cites W2167121797 @default.
• W2043774268 cites W2170611362 @default.
• W2043774268 doi "https://doi.org/10.1016/j.dnarep.2012.07.004" @default.
• W2043774268 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22917548" @default.
• W2043774268 hasPublicationYear "2012" @default.
• W2043774268 type Work @default.
• W2043774268 sameAs 2043774268 @default.
• W2043774268 citedByCount "3" @default.
• W2043774268 countsByYear W20437742682015 @default.
• W2043774268 countsByYear W20437742682017 @default.
• W2043774268 countsByYear W20437742682019 @default.
• W2043774268 crossrefType "journal-article" @default.
• W2043774268 hasAuthorship W2043774268A5032296502 @default.
• W2043774268 hasAuthorship W2043774268A5034608225 @default.
• W2043774268 hasConcept C102744134 @default.
• W2043774268 hasConcept C104317684 @default.
• W2043774268 hasConcept C130965112 @default.
• W2043774268 hasConcept C134935766 @default.
• W2043774268 hasConcept C143065580 @default.
• W2043774268 hasConcept C153911025 @default.
• W2043774268 hasConcept C2777271071 @default.
• W2043774268 hasConcept C2777576037 @default.
• W2043774268 hasConcept C2778041695 @default.
• W2043774268 hasConcept C2779222958 @default.
• W2043774268 hasConcept C54355233 @default.
• W2043774268 hasConcept C552990157 @default.
• W2043774268 hasConcept C86803240 @default.
• W2043774268 hasConcept C95444343 @default.
• W2043774268 hasConceptScore W2043774268C102744134 @default.
• W2043774268 hasConceptScore W2043774268C104317684 @default.
• W2043774268 hasConceptScore W2043774268C130965112 @default.
• W2043774268 hasConceptScore W2043774268C134935766 @default.
• W2043774268 hasConceptScore W2043774268C143065580 @default.
• W2043774268 hasConceptScore W2043774268C153911025 @default.
• W2043774268 hasConceptScore W2043774268C2777271071 @default.
• W2043774268 hasConceptScore W2043774268C2777576037 @default.
• W2043774268 hasConceptScore W2043774268C2778041695 @default.
• W2043774268 hasConceptScore W2043774268C2779222958 @default.
• W2043774268 hasConceptScore W2043774268C54355233 @default.
• W2043774268 hasConceptScore W2043774268C552990157 @default.
• W2043774268 hasConceptScore W2043774268C86803240 @default.
• W2043774268 hasConceptScore W2043774268C95444343 @default.
• W2043774268 hasIssue "10" @default.
• W2043774268 hasLocation W20437742681 @default.
• W2043774268 hasLocation W20437742682 @default.
• W2043774268 hasOpenAccess W2043774268 @default.
• W2043774268 hasPrimaryLocation W20437742681 @default.
• W2043774268 hasRelatedWork W1973112089 @default.
• W2043774268 hasRelatedWork W2036682748 @default.

• next