Matches in SemOpenAlex for { <https://semopenalex.org/work/W2043796829> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W2043796829 endingPage "1295" @default.
- W2043796829 startingPage "1291" @default.
- W2043796829 abstract "We have examined the interaction of the estradiol receptor molecule with chromatin in MCF-7 cells, a human breast tumor cell line responsive to estradiol. Receptor was found associated with the various nucleosomal products produced by digestion with micrococcal nuclease. In order to determine whether these receptor binding sites were distributed in a random or nonrandom manner within the chromatin, we have fractionated MCF-7 cell chromatin into transcriptionally active and inactive fractions by limited micrococcal nuclease digestion followed by Mg(2+) precipitation. A comparison of the Mg(2+)-soluble and insoluble chromatin fractions showed that the Mg(2+)-soluble fraction: (i) was composed predominantly of mononucleosomes; (ii) was enriched in nonhistone proteins; (iii) apparently lacked histone H1; (iv) was enriched approximately 5-fold in transcribed sequences as measured by a cDNA probe to cytoplasmic poly(A)-RNA sequences; and (v) was depleted at least 5-fold of globin sequences, which is presumably a nontranscribed gene in these cells. When these cells were stimulated with beta-[(3)H]estradiol, the Mg(2+)-soluble fraction showed a significant enrichment in chromatin-bound estradiol receptor: the Mg(2+)-soluble mononucleosomes showed a 3- to 4-fold enrichment and the di- and trinucleosomes, a 7- to 19-fold enrichment, when compared to the corresponding subunits in the Mg(2+)-insoluble chromatin fraction. This cofractionation of chromatin enriched in transcribed sequences and bound estradiol receptor indicated that receptor binding to MCF-7 cell chromatin was not random but, rather, occurred preferentially in specific regions of the chromatin." @default.
- W2043796829 created "2016-06-24" @default.
- W2043796829 creator A5048116502 @default.
- W2043796829 creator A5065004209 @default.
- W2043796829 date "1980-03-01" @default.
- W2043796829 modified "2023-10-16" @default.
- W2043796829 title "Enrichment of estradiol-receptor complexes in a transcriptionally active fraction of chromatin from MCF-7 cells" @default.
- W2043796829 cites W1484760080 @default.
- W2043796829 cites W1490750970 @default.
- W2043796829 cites W1495332006 @default.
- W2043796829 cites W1501788884 @default.
- W2043796829 cites W1525730550 @default.
- W2043796829 cites W1602555107 @default.
- W2043796829 cites W1607004775 @default.
- W2043796829 cites W1675332423 @default.
- W2043796829 cites W172854789 @default.
- W2043796829 cites W1811094195 @default.
- W2043796829 cites W1954288537 @default.
- W2043796829 cites W1966439005 @default.
- W2043796829 cites W1969875683 @default.
- W2043796829 cites W1977364026 @default.
- W2043796829 cites W1979409535 @default.
- W2043796829 cites W1980894884 @default.
- W2043796829 cites W1992502560 @default.
- W2043796829 cites W2009106729 @default.
- W2043796829 cites W2019725919 @default.
- W2043796829 cites W2031935887 @default.
- W2043796829 cites W2038776093 @default.
- W2043796829 cites W2041923106 @default.
- W2043796829 cites W2065790268 @default.
- W2043796829 cites W2065979034 @default.
- W2043796829 cites W2066160869 @default.
- W2043796829 cites W2078825043 @default.
- W2043796829 cites W2084324725 @default.
- W2043796829 cites W2091738072 @default.
- W2043796829 cites W2103283518 @default.
- W2043796829 cites W2139026276 @default.
- W2043796829 cites W2141981573 @default.
- W2043796829 cites W2162947994 @default.
- W2043796829 cites W2167885697 @default.
- W2043796829 cites W2278354703 @default.
- W2043796829 doi "https://doi.org/10.1073/pnas.77.3.1291" @default.
- W2043796829 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/348480" @default.
- W2043796829 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6929487" @default.
- W2043796829 hasPublicationYear "1980" @default.
- W2043796829 type Work @default.
- W2043796829 sameAs 2043796829 @default.
- W2043796829 citedByCount "35" @default.
- W2043796829 crossrefType "journal-article" @default.
- W2043796829 hasAuthorship W2043796829A5048116502 @default.
- W2043796829 hasAuthorship W2043796829A5065004209 @default.
- W2043796829 hasBestOaLocation W20437968291 @default.
- W2043796829 hasConcept C101152365 @default.
- W2043796829 hasConcept C139024627 @default.
- W2043796829 hasConcept C153911025 @default.
- W2043796829 hasConcept C170493617 @default.
- W2043796829 hasConcept C552990157 @default.
- W2043796829 hasConcept C55493867 @default.
- W2043796829 hasConcept C64927066 @default.
- W2043796829 hasConcept C83640560 @default.
- W2043796829 hasConcept C84772758 @default.
- W2043796829 hasConcept C86803240 @default.
- W2043796829 hasConceptScore W2043796829C101152365 @default.
- W2043796829 hasConceptScore W2043796829C139024627 @default.
- W2043796829 hasConceptScore W2043796829C153911025 @default.
- W2043796829 hasConceptScore W2043796829C170493617 @default.
- W2043796829 hasConceptScore W2043796829C552990157 @default.
- W2043796829 hasConceptScore W2043796829C55493867 @default.
- W2043796829 hasConceptScore W2043796829C64927066 @default.
- W2043796829 hasConceptScore W2043796829C83640560 @default.
- W2043796829 hasConceptScore W2043796829C84772758 @default.
- W2043796829 hasConceptScore W2043796829C86803240 @default.
- W2043796829 hasIssue "3" @default.
- W2043796829 hasLocation W20437968291 @default.
- W2043796829 hasLocation W20437968292 @default.
- W2043796829 hasLocation W20437968293 @default.
- W2043796829 hasLocation W20437968294 @default.
- W2043796829 hasOpenAccess W2043796829 @default.
- W2043796829 hasPrimaryLocation W20437968291 @default.
- W2043796829 hasRelatedWork W1992707715 @default.
- W2043796829 hasRelatedWork W2016891566 @default.
- W2043796829 hasRelatedWork W2022534851 @default.
- W2043796829 hasRelatedWork W2030121240 @default.
- W2043796829 hasRelatedWork W2042740750 @default.
- W2043796829 hasRelatedWork W2059718302 @default.
- W2043796829 hasRelatedWork W2062023201 @default.
- W2043796829 hasRelatedWork W2067251944 @default.
- W2043796829 hasRelatedWork W2069179516 @default.
- W2043796829 hasRelatedWork W2185911465 @default.
- W2043796829 hasVolume "77" @default.
- W2043796829 isParatext "false" @default.
- W2043796829 isRetracted "false" @default.
- W2043796829 magId "2043796829" @default.
- W2043796829 workType "article" @default.