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- W2043930164 abstract "A431 cells grew in protein-free Coon's modified Ham's F12 medium at a similar rate to that in medium supplemented with calf serum and secreted a growth factor capable of stimulating DNA synthesis in BALB/c3T3 cells. This factor had strong affinity for heparin and was partially purified from the conditioned medium by heparin-Sepharose affinity chromatography and molecular sieving on Bio-Gel P-60. The apparent molecular weight of the factor was 20-30K. Its activity was inhibited by heparin at concentrations of above 0.03 microgram/ml." @default.
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- W2043930164 date "1987-05-01" @default.
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- W2043930164 title "Secretion of DNA synthesis factor (DSF) by A431 cells that can grow in protein-free medium" @default.
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- W2043930164 doi "https://doi.org/10.1016/0309-1651(87)90002-6" @default.
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