FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2043939637> ?p ?o ?g. }

• W2043939637 endingPage "2083" @default.
• W2043939637 startingPage "2081" @default.
• W2043939637 abstract "We previously established a mouse pancreatic stem cell line without genetic manipulation. In this study, we sought to identify and isolate human pancreatic stem/progenitor cells. We also tested whether growth factors and protein transduction of pancreatic and duodenal homeobox factor-1 (PDX-1) and BETA2/NeuroD into human pancreatic stem/progenitor cells induced insulin or pancreas-related gene expressions.Human pancreata from brain-dead donors were used for islet isolation with the standard Ricordi technique modified by the Edmonton protocol. The cells from a duct-rich population were cultured in several media, based on those designed for mouse pancreatic or for human embryonic stem cells. To induce cell differentiation, cells were cultured for 2 weeks with exendin-4, nicotinamide, keratinocyte growth factor, PDX-1 protein, or BETA2/NeuroD protein.The cells in serum-free media showed morphologies similar to a mouse pancreatic stem cell line, while the cells in the medium for human embryonic stem cells formed fibroblast-like morphologies. The nucleus/cytoplasm ratios of the cells in each culture medium decreased during the culture. The cells stopped dividing after 30 days, suggesting that they had entered senescence. The cells treated with induction medium differentiated into insulin-producing cells, expressing pancreas-related genes.Duplications of cells from a duct-rich population were limited. Induction therapy with several growth factors and transduction proteins might provide a potential new strategy for induction of transplantable insulin-producing cells." @default.
• W2043939637 created "2016-06-24" @default.
• W2043939637 creator A5000633937 @default.
• W2043939637 creator A5002432921 @default.
• W2043939637 creator A5003021411 @default.
• W2043939637 creator A5009641276 @default.
• W2043939637 creator A5012957428 @default.
• W2043939637 creator A5013965216 @default.
• W2043939637 creator A5015411015 @default.
• W2043939637 creator A5017433482 @default.
• W2043939637 creator A5019865591 @default.
• W2043939637 creator A5021126434 @default.
• W2043939637 creator A5024434636 @default.
• W2043939637 creator A5029686628 @default.
• W2043939637 creator A5037027421 @default.
• W2043939637 creator A5058185889 @default.
• W2043939637 creator A5073576456 @default.
• W2043939637 creator A5078861466 @default.
• W2043939637 date "2010-07-01" @default.
• W2043939637 modified "2023-09-28" @default.
• W2043939637 title "Induction of Insulin-Producing Cells From Human Pancreatic Progenitor Cells" @default.
• W2043939637 cites W2004063914 @default.
• W2043939637 cites W2016193795 @default.
• W2043939637 cites W2027768678 @default.
• W2043939637 cites W2065567658 @default.
• W2043939637 cites W2078722467 @default.
• W2043939637 cites W2081305200 @default.
• W2043939637 cites W2100166470 @default.
• W2043939637 cites W2108380410 @default.
• W2043939637 cites W2108841123 @default.
• W2043939637 cites W2133902171 @default.
• W2043939637 cites W2137794809 @default.
• W2043939637 cites W2146383332 @default.
• W2043939637 cites W2154532011 @default.
• W2043939637 cites W2169335085 @default.
• W2043939637 cites W2171678230 @default.
• W2043939637 cites W2328786770 @default.
• W2043939637 cites W40259379 @default.
• W2043939637 doi "https://doi.org/10.1016/j.transproceed.2010.05.097" @default.
• W2043939637 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20692413" @default.
• W2043939637 hasPublicationYear "2010" @default.
• W2043939637 type Work @default.
• W2043939637 sameAs 2043939637 @default.
• W2043939637 citedByCount "17" @default.
• W2043939637 countsByYear W20439396372012 @default.
• W2043939637 countsByYear W20439396372013 @default.
• W2043939637 countsByYear W20439396372014 @default.
• W2043939637 countsByYear W20439396372015 @default.
• W2043939637 countsByYear W20439396372016 @default.
• W2043939637 countsByYear W20439396372017 @default.
• W2043939637 countsByYear W20439396372018 @default.
• W2043939637 countsByYear W20439396372020 @default.
• W2043939637 countsByYear W20439396372021 @default.
• W2043939637 crossrefType "journal-article" @default.
• W2043939637 hasAuthorship W2043939637A5000633937 @default.
• W2043939637 hasAuthorship W2043939637A5002432921 @default.
• W2043939637 hasAuthorship W2043939637A5003021411 @default.
• W2043939637 hasAuthorship W2043939637A5009641276 @default.
• W2043939637 hasAuthorship W2043939637A5012957428 @default.
• W2043939637 hasAuthorship W2043939637A5013965216 @default.
• W2043939637 hasAuthorship W2043939637A5015411015 @default.
• W2043939637 hasAuthorship W2043939637A5017433482 @default.
• W2043939637 hasAuthorship W2043939637A5019865591 @default.
• W2043939637 hasAuthorship W2043939637A5021126434 @default.
• W2043939637 hasAuthorship W2043939637A5024434636 @default.
• W2043939637 hasAuthorship W2043939637A5029686628 @default.
• W2043939637 hasAuthorship W2043939637A5037027421 @default.
• W2043939637 hasAuthorship W2043939637A5058185889 @default.
• W2043939637 hasAuthorship W2043939637A5073576456 @default.
• W2043939637 hasAuthorship W2043939637A5078861466 @default.
• W2043939637 hasConcept C104317684 @default.
• W2043939637 hasConcept C123012128 @default.
• W2043939637 hasConcept C126322002 @default.
• W2043939637 hasConcept C134018914 @default.
• W2043939637 hasConcept C145103041 @default.
• W2043939637 hasConcept C148738053 @default.
• W2043939637 hasConcept C201750760 @default.
• W2043939637 hasConcept C202751555 @default.
• W2043939637 hasConcept C20875687 @default.
• W2043939637 hasConcept C2778764654 @default.
• W2043939637 hasConcept C2778994924 @default.
• W2043939637 hasConcept C28328180 @default.
• W2043939637 hasConcept C2908647359 @default.
• W2043939637 hasConcept C54355233 @default.
• W2043939637 hasConcept C71924100 @default.
• W2043939637 hasConcept C86803240 @default.
• W2043939637 hasConcept C95444343 @default.
• W2043939637 hasConcept C99454951 @default.
• W2043939637 hasConceptScore W2043939637C104317684 @default.
• W2043939637 hasConceptScore W2043939637C123012128 @default.
• W2043939637 hasConceptScore W2043939637C126322002 @default.
• W2043939637 hasConceptScore W2043939637C134018914 @default.
• W2043939637 hasConceptScore W2043939637C145103041 @default.
• W2043939637 hasConceptScore W2043939637C148738053 @default.
• W2043939637 hasConceptScore W2043939637C201750760 @default.
• W2043939637 hasConceptScore W2043939637C202751555 @default.
• W2043939637 hasConceptScore W2043939637C20875687 @default.
• W2043939637 hasConceptScore W2043939637C2778764654 @default.

• next