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• W2043940993 abstract "Abstract Glucose-grown cells of Streptococcus salivarius have been shown to contain a polyglucose phosphorylase which had maximum activity in the stationary phase of growth. Despite the fact that activity in crude cell-free extracts was two- to threefold greater in the presence of corn dextrin than with oyster glycogen, subsequent purification (200-fold) of the enzyme from the soluble fraction of the organism by protamine sulfate treatment, ammonium sulfate fractionation (30–50%), ion exchange chromatography on DEAE-cellulose and gel filtration on Sephadex G-200 demonstrated that this dextrin/glycogen activity was associated with a single enzyme. Since glucose-grown cells of S. salivarius are known to synthesize a typical glycogen polymer, the enzyme was named: glycogen phosphorylase. The purified enzyme preparation was devoid of phosphoglucomutase and ADP-glucose pyrophosphorylase, but contained a small amount of ADP-glucose: α-1,4 glucan transferase activity. The enzyme was stable at −10 °C in the presence of 0.2 m NaF, while the pH optimum for the enzyme was 6.0 both with glycogen and with dextrin. With the purified enzyme, corn dextrin was the best primer, both in the direction of synthesis and in the direction of phosphorolysis, being 1.8–1.9 times more effective than purified S. salivarius glycogen. When the enzyme was assayed in the direction of glycogen synthesis, a K m value of 3.4 m m was obtained for glucose-1- P , while the values for S. salivarius glycogen, oyster glycogen and corn dextrin were 25, 42, and 40 mg/ml, respectively. In the direction of phosphorolysis, K m values were 20 m m for P i obtained with oyster glycogen, 25 m m for P i with corn dextrin, and 20 mg/ml and 26 mg/ml for oyster glycogen and corn dextrin, respectively. Present data suggests no involvement of -SH groups in enzyme catalysis, while the enzyme was inhibited by divalent ions with the severest inhibition being observed with Ca 2+ , Zn 2+ and Fe 2+ . The two ion chelators, EDTA and EGTA, had no effect on enzyme activity." @default.
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• W2043940993 date "1973-01-01" @default.
• W2043940993 modified "2023-09-26" @default.
• W2043940993 title "Purification and properties of glycogen phosphorylase from Streptococcus salivarius" @default.
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• W2043940993 doi "https://doi.org/10.1016/0003-9861(73)90061-1" @default.
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