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- W2043965162 abstract "Of 77 patients with bladder carcinoma, 99 tissue specimens—including tissues of patients with recurrent tumors taken after radiotherapy or cytostatics—were subjected to chromosomal analysis. In 42 specimens, recognizable metaphases could be obtained after conventional Giemsa staining and in a smaller number after C- and/or G-banding. All except one had abnormalities of the chromosomes. Short-term cultures for 24–48 hr in RPMI 1640 plus 15% fetal calf serum plus penicillin-streptomycin gave better results than a direct technique (30 min in 0.075 M KCl + 0.1 μg colcemid/ml at 37°C, followed by fixation). In low stage/grade tumors the number of recognizable metaphases obtained after short-term cultures is lower than in higher stage/grade tissue specimens." @default.
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- W2043965162 date "1985-04-01" @default.
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- W2043965162 title "Chromosomal analysis of bladder cancer: Technical aspects" @default.
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- W2043965162 doi "https://doi.org/10.1016/0165-4608(85)90053-6" @default.
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