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- W2043972155 abstract "Specific LexA cleavage can occur under two different conditions: RecA-mediated cleavage requires an activated form of RecA, while an intramolecular self-cleavage termed autodigestion proceeds spontaneously at high pH and does not involved RecA. The two cleavage reactions are closely related. We postulate that RecA stimulates autodigestion rather than acting as a typical protease, and it is proposed to term this activity ‘RecA coprotease’ to emphasize this indirect role. The mechanism of autodigestion is similar to that of a serine protease, and RecA appears to act by reducing the pKa of a critical lysine residue LexA. A new class of mutants, termed lexA (IndS), is described; these mutations increase the rate of LexA cleavage." @default.
- W2043972155 created "2016-06-24" @default.
- W2043972155 creator A5018548788 @default.
- W2043972155 date "1991-04-01" @default.
- W2043972155 modified "2023-10-11" @default.
- W2043972155 title "Mechanism of specific LexA cleavage: Autodigestion and the role of RecA coprotease" @default.
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- W2043972155 doi "https://doi.org/10.1016/0300-9084(91)90108-d" @default.
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