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- W204397595 abstract "Abnormal behavior and impaired brain function have been attributed to zinc deficiency in experimental animals. The bulk measurement of zinc in various brain parts indicated that this element was highly conserved during zinc deficiency. In this study proton-induced x-ray fluorescence (PIXE) was used to measure and localize zinc in thin brain sections of growth in rats maintained on a zinc-deficient diet (<1 ppm zinc). The zinc-supplemented control animals were fed the same diet with 25 ppm zinc-supplemented water. After 30 days of this regimen the rats were perfused with 0.85% saline until the brains were cleared of blood. The brains were excised, rapidly frozen, and 30-..mu..m thick sections were cut with a stainless steel microtome knife. These sections were backed with adhesive tape and placed in a 50-..mu..m diameter, 2.5-MeV proton beam, and the characteristic K x-rays produced were measured with a Si (Li) x-ray detector. The average brain zinc concentration measured by microPIXE was similar to values obtained from bulk samples with plasma emission spectrometry. There appeared to be a slightly higher concentration of zinc in basal ganglia, cortex, and white matter of zinc-deficient rat as compared to zinc-supplemented controls. The overall brain zinc concentration appeared to be relativelymore » unaffected by zinc deficiency.« less" @default.
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- W204397595 date "1986-03-05" @default.
- W204397595 modified "2023-09-26" @default.
- W204397595 title "Microlocalization of brain zinc in zinc-deficient rats" @default.
- W204397595 hasPublicationYear "1986" @default.
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