Matches in SemOpenAlex for { <https://semopenalex.org/work/W2043982016> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W2043982016 endingPage "161" @default.
- W2043982016 startingPage "155" @default.
- W2043982016 abstract "In this study, we have spin-labeled the lysine and cysteine residues of low-density lipoprotein (LDL) using N-4-(2,2,6,6-tetramethylpiperidinyl-1-oxyl-4-yl) maleimide (MAL-6) and succinimidyl-2,2,5,5-tetramethyl-3-pyrroline-1-oxyl-3-carboxylate (SSL), respectively. The electron spin resonance (ESR) spectrum of SSL bound to LDL indicated that the nitroxide moiety was relatively mobile. In contrast, the ESR spectrum of MAL-6 bound to LDL showed that the nitroxide moiety was rotationally restricted. Using the continuous-wave power saturation technique in the presence of hydrophobic and hydrophilic paramagnetic relaxing agents, we have determined that (i) approximately 60-70% of lysine-bound SSL is exposed to the aqueous phase, (ii) approximately 30-40% of SSL-LDL is buried in a hydrophobic region, and (iii) MAL-6 bound to LDL is localized predominantly in the hydrophobic region. During Cu(2+)-initiated oxidation of spin-labeled LDL, nitroxide labels located in a hydrophobic environment were predominantly degraded. Nitroxide destruction was inhibited by butylated hydroxytoluene, indicating the role of lipid peroxidation in this process. ESR data also showed that Cu2+ binding to lysine is essential for LDL oxidation. The spin label methodology may be useful for the investigation of site-specific radical reactions in LDL." @default.
- W2043982016 created "2016-06-24" @default.
- W2043982016 creator A5024996281 @default.
- W2043982016 creator A5035372773 @default.
- W2043982016 creator A5059881003 @default.
- W2043982016 creator A5063258499 @default.
- W2043982016 creator A5077857077 @default.
- W2043982016 date "1995-06-01" @default.
- W2043982016 modified "2023-09-23" @default.
- W2043982016 title "Spin-Labeling Study of the Oxidative Damage to Low-Density Lipoprotein" @default.
- W2043982016 doi "https://doi.org/10.1006/abbi.1995.1354" @default.
- W2043982016 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7793976" @default.
- W2043982016 hasPublicationYear "1995" @default.
- W2043982016 type Work @default.
- W2043982016 sameAs 2043982016 @default.
- W2043982016 citedByCount "28" @default.
- W2043982016 countsByYear W20439820162019 @default.
- W2043982016 crossrefType "journal-article" @default.
- W2043982016 hasAuthorship W2043982016A5024996281 @default.
- W2043982016 hasAuthorship W2043982016A5035372773 @default.
- W2043982016 hasAuthorship W2043982016A5059881003 @default.
- W2043982016 hasAuthorship W2043982016A5063258499 @default.
- W2043982016 hasAuthorship W2043982016A5077857077 @default.
- W2043982016 hasConcept C111998727 @default.
- W2043982016 hasConcept C121332964 @default.
- W2043982016 hasConcept C139066938 @default.
- W2043982016 hasConcept C15920480 @default.
- W2043982016 hasConcept C169912494 @default.
- W2043982016 hasConcept C178790620 @default.
- W2043982016 hasConcept C181199279 @default.
- W2043982016 hasConcept C185592680 @default.
- W2043982016 hasConcept C187961010 @default.
- W2043982016 hasConcept C2776142628 @default.
- W2043982016 hasConcept C2776568683 @default.
- W2043982016 hasConcept C2778004101 @default.
- W2043982016 hasConcept C2778163477 @default.
- W2043982016 hasConcept C2779201268 @default.
- W2043982016 hasConcept C2779620165 @default.
- W2043982016 hasConcept C2779661893 @default.
- W2043982016 hasConcept C41625074 @default.
- W2043982016 hasConcept C46141821 @default.
- W2043982016 hasConcept C521977710 @default.
- W2043982016 hasConcept C55493867 @default.
- W2043982016 hasConcept C71240020 @default.
- W2043982016 hasConcept C75473681 @default.
- W2043982016 hasConcept C79123411 @default.
- W2043982016 hasConcept C8010536 @default.
- W2043982016 hasConceptScore W2043982016C111998727 @default.
- W2043982016 hasConceptScore W2043982016C121332964 @default.
- W2043982016 hasConceptScore W2043982016C139066938 @default.
- W2043982016 hasConceptScore W2043982016C15920480 @default.
- W2043982016 hasConceptScore W2043982016C169912494 @default.
- W2043982016 hasConceptScore W2043982016C178790620 @default.
- W2043982016 hasConceptScore W2043982016C181199279 @default.
- W2043982016 hasConceptScore W2043982016C185592680 @default.
- W2043982016 hasConceptScore W2043982016C187961010 @default.
- W2043982016 hasConceptScore W2043982016C2776142628 @default.
- W2043982016 hasConceptScore W2043982016C2776568683 @default.
- W2043982016 hasConceptScore W2043982016C2778004101 @default.
- W2043982016 hasConceptScore W2043982016C2778163477 @default.
- W2043982016 hasConceptScore W2043982016C2779201268 @default.
- W2043982016 hasConceptScore W2043982016C2779620165 @default.
- W2043982016 hasConceptScore W2043982016C2779661893 @default.
- W2043982016 hasConceptScore W2043982016C41625074 @default.
- W2043982016 hasConceptScore W2043982016C46141821 @default.
- W2043982016 hasConceptScore W2043982016C521977710 @default.
- W2043982016 hasConceptScore W2043982016C55493867 @default.
- W2043982016 hasConceptScore W2043982016C71240020 @default.
- W2043982016 hasConceptScore W2043982016C75473681 @default.
- W2043982016 hasConceptScore W2043982016C79123411 @default.
- W2043982016 hasConceptScore W2043982016C8010536 @default.
- W2043982016 hasIssue "1" @default.
- W2043982016 hasLocation W20439820161 @default.
- W2043982016 hasLocation W20439820162 @default.
- W2043982016 hasOpenAccess W2043982016 @default.
- W2043982016 hasPrimaryLocation W20439820161 @default.
- W2043982016 hasRelatedWork W1450052830 @default.
- W2043982016 hasRelatedWork W1969884510 @default.
- W2043982016 hasRelatedWork W1977506701 @default.
- W2043982016 hasRelatedWork W2043982016 @default.
- W2043982016 hasRelatedWork W2058620782 @default.
- W2043982016 hasRelatedWork W2079852184 @default.
- W2043982016 hasRelatedWork W2150222677 @default.
- W2043982016 hasRelatedWork W2537233966 @default.
- W2043982016 hasRelatedWork W2908419253 @default.
- W2043982016 hasRelatedWork W3029691803 @default.
- W2043982016 hasVolume "320" @default.
- W2043982016 isParatext "false" @default.
- W2043982016 isRetracted "false" @default.
- W2043982016 magId "2043982016" @default.
- W2043982016 workType "article" @default.