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- W2043989787 abstract "A simple method has been developed for the coupling of oligosaccharides to Sepharose. The sugars are reacted with β-(p-aminophenyl)-ethylamine to form N-alkylglycosides which are then reduced with sodium borohydride to stable secondary amines. The derivatives are then coupled to cyanogen bromide-activated Sepharose through their arylamino groups. Yields are essentially quantitative based on starting oligosaccharides. An affinity column containing lacto-N-difucohexaose I coupled to Sepharose by this method was used for the purification of an antibody directed against this oligosaccharide. The antibody is absorbed by the gel and is specifically eluted by the free sugar." @default.
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- W2043989787 date "1975-02-01" @default.
- W2043989787 modified "2023-10-14" @default.
- W2043989787 title "Affinity chromatography of carbohydrate-specific immunoglobulins: Coupling of oligosaccharides to Sepharose" @default.
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- W2043989787 doi "https://doi.org/10.1016/0006-291x(75)90442-8" @default.
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