Matches in SemOpenAlex for { <https://semopenalex.org/work/W2044017071> ?p ?o ?g. }
- W2044017071 endingPage "10130" @default.
- W2044017071 startingPage "10125" @default.
- W2044017071 abstract "The kinetic mechanism of plastocyanin oxidation by photosystem I in the cyanobacterium Synechocystis sp. PCC 6803 is drastically changed by modifying the metalloprotein by site-directed mutagenesis. The mutations herein considered concern four specific residues, two in the east face and the other two in the hydrophobic patch of plastocyanin. The first set of mutants include D44A, D44K, D47A, and D47R, as well as the double mutants D44A/D47A and D44R/D47R; the second set consists of L12A and K33E. The kinetic efficiency of all these mutant plastocyanins has been analyzed by laser-flash absorption spectroscopy. The plastocyanin concentration dependence of the observed electron transfer rate constant (kobs) is linear with most mutant plastocyanins, as with wild-type plastocyanin, but exhibits a saturation plateau at high protein concentration with the double mutant D44R/D47R, which suggests the formation of a plastocyanin-PSI transient complex. The effect of ionic strength on kobs varies from the wild-type plastocyanin to some of the mutants, for instance D44K, for which the salt concentration dependence of kobs is just the reverse as compared to the wild-type protein. The ionic strength dependence of kobs with D44R/D47R exhibits a bell-shaped profile, which is similar to that of green algae and higher plants. These findings indicate that the double mutant D44R/D47R follows a reaction mechanism involving not only complex formation with PSI but also further reorientation to properly accommodate the redox centers prior to electron transfer, as is the case in most evolved species, whereas the wild-type copper protein reacts with PSI by following a simple collisional kinetic model." @default.
- W2044017071 created "2016-06-24" @default.
- W2044017071 creator A5049125752 @default.
- W2044017071 creator A5058167768 @default.
- W2044017071 creator A5062143235 @default.
- W2044017071 creator A5075917705 @default.
- W2044017071 date "1997-08-01" @default.
- W2044017071 modified "2023-10-16" @default.
- W2044017071 title "Changes in the Reaction Mechanism of Electron Transfer from Plastocyanin to Photosystem I in the Cyanobacterium <i>Synechocystis </i>sp. PCC 6803 As Induced by Site-Directed Mutagenesis of the Copper Protein" @default.
- W2044017071 cites W1538055033 @default.
- W2044017071 cites W1570155960 @default.
- W2044017071 cites W1829062329 @default.
- W2044017071 cites W1967006909 @default.
- W2044017071 cites W1974907033 @default.
- W2044017071 cites W1990197565 @default.
- W2044017071 cites W1996062078 @default.
- W2044017071 cites W2028906717 @default.
- W2044017071 cites W2033088431 @default.
- W2044017071 cites W2038093030 @default.
- W2044017071 cites W2056307169 @default.
- W2044017071 cites W2062411819 @default.
- W2044017071 cites W2074779648 @default.
- W2044017071 cites W2152639174 @default.
- W2044017071 doi "https://doi.org/10.1021/bi9708601" @default.
- W2044017071 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9254609" @default.
- W2044017071 hasPublicationYear "1997" @default.
- W2044017071 type Work @default.
- W2044017071 sameAs 2044017071 @default.
- W2044017071 citedByCount "37" @default.
- W2044017071 countsByYear W20440170712013 @default.
- W2044017071 countsByYear W20440170712014 @default.
- W2044017071 countsByYear W20440170712016 @default.
- W2044017071 countsByYear W20440170712018 @default.
- W2044017071 countsByYear W20440170712020 @default.
- W2044017071 countsByYear W20440170712021 @default.
- W2044017071 crossrefType "journal-article" @default.
- W2044017071 hasAuthorship W2044017071A5049125752 @default.
- W2044017071 hasAuthorship W2044017071A5058167768 @default.
- W2044017071 hasAuthorship W2044017071A5062143235 @default.
- W2044017071 hasAuthorship W2044017071A5075917705 @default.
- W2044017071 hasConcept C104317684 @default.
- W2044017071 hasConcept C123669783 @default.
- W2044017071 hasConcept C12554922 @default.
- W2044017071 hasConcept C143065580 @default.
- W2044017071 hasConcept C147789679 @default.
- W2044017071 hasConcept C154828652 @default.
- W2044017071 hasConcept C178790620 @default.
- W2044017071 hasConcept C181312439 @default.
- W2044017071 hasConcept C183688256 @default.
- W2044017071 hasConcept C184651966 @default.
- W2044017071 hasConcept C185592680 @default.
- W2044017071 hasConcept C187428577 @default.
- W2044017071 hasConcept C188231169 @default.
- W2044017071 hasConcept C2778574204 @default.
- W2044017071 hasConcept C2780238584 @default.
- W2044017071 hasConcept C544778455 @default.
- W2044017071 hasConcept C55493867 @default.
- W2044017071 hasConcept C75473681 @default.
- W2044017071 hasConcept C80298142 @default.
- W2044017071 hasConcept C86803240 @default.
- W2044017071 hasConcept C96806796 @default.
- W2044017071 hasConceptScore W2044017071C104317684 @default.
- W2044017071 hasConceptScore W2044017071C123669783 @default.
- W2044017071 hasConceptScore W2044017071C12554922 @default.
- W2044017071 hasConceptScore W2044017071C143065580 @default.
- W2044017071 hasConceptScore W2044017071C147789679 @default.
- W2044017071 hasConceptScore W2044017071C154828652 @default.
- W2044017071 hasConceptScore W2044017071C178790620 @default.
- W2044017071 hasConceptScore W2044017071C181312439 @default.
- W2044017071 hasConceptScore W2044017071C183688256 @default.
- W2044017071 hasConceptScore W2044017071C184651966 @default.
- W2044017071 hasConceptScore W2044017071C185592680 @default.
- W2044017071 hasConceptScore W2044017071C187428577 @default.
- W2044017071 hasConceptScore W2044017071C188231169 @default.
- W2044017071 hasConceptScore W2044017071C2778574204 @default.
- W2044017071 hasConceptScore W2044017071C2780238584 @default.
- W2044017071 hasConceptScore W2044017071C544778455 @default.
- W2044017071 hasConceptScore W2044017071C55493867 @default.
- W2044017071 hasConceptScore W2044017071C75473681 @default.
- W2044017071 hasConceptScore W2044017071C80298142 @default.
- W2044017071 hasConceptScore W2044017071C86803240 @default.
- W2044017071 hasConceptScore W2044017071C96806796 @default.
- W2044017071 hasIssue "33" @default.
- W2044017071 hasLocation W20440170711 @default.
- W2044017071 hasLocation W20440170712 @default.
- W2044017071 hasOpenAccess W2044017071 @default.
- W2044017071 hasPrimaryLocation W20440170711 @default.
- W2044017071 hasRelatedWork W110157579 @default.
- W2044017071 hasRelatedWork W1604485725 @default.
- W2044017071 hasRelatedWork W1992668400 @default.
- W2044017071 hasRelatedWork W2033037545 @default.
- W2044017071 hasRelatedWork W2044017071 @default.
- W2044017071 hasRelatedWork W2151476768 @default.
- W2044017071 hasRelatedWork W2264423150 @default.
- W2044017071 hasRelatedWork W2497937963 @default.
- W2044017071 hasRelatedWork W294034645 @default.
- W2044017071 hasRelatedWork W611710906 @default.
- W2044017071 hasVolume "36" @default.