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- W2044111978 abstract "The molecular reasons for the sensitivity of microbial lipases towards acetaldehyde, emerging as unavoidable by-product from acyl transfer reactions employing vinyl esters as acyl donors, were shown to be associated with specific properties of lysine residues. Since the mechanism of deactivation involves the formation of Schiff bases at the lysine ε-amino groups, the relative reactivity (i.e. nucleophilicity) of each residue was estimated by using an electronic (pKa value) and a steric parameter (accessible surface area of the side chain). Sensitive lipases, as from Candida rugosa and Geotrichum candidum, possess several lysine residues that have high pKa values (> 12) and are highly exposed to the solvent (surface areas of 210–220 Å2). In contrast, the lysine groups of stable lipases like from Rhizomucor miehei, Candida antarctica B and Pseudomonas glumae have moderate pKa values (up to 11.6) and are rather buried (surface areas of 130–150 Å2). A close investigation of Candida rugosa lipase revealed that the most exposed lysine residues are located in the lid region (Lys75 and Lys85). The data suggest that Lys75, which is involved in fixing the lid in its open conformation, is presumably the prime target for deactivation by acetaldehyde." @default.
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- W2044111978 date "1997-06-01" @default.
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- W2044111978 title "Molecular reasons for lipase-sensitivity against acetaldehyde" @default.
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- W2044111978 doi "https://doi.org/10.1016/s1381-1177(96)00045-8" @default.
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