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• W2044112600 abstract "Bulge structure is one of the well-known and important nucleic acid secondary structures containing unpaired nucleotides. Although the thermodynamic property has been thoroughly investigated, the detailed kinetic property of the bulged helix formation is still unknown. We now investigated the helix formation mechanism for bulged helices using temperature-jump experiments. The activation energy for the duplex association (Ea+1) obtained from the temperature dependence of the rate constants showed that the Ea+1 value depended on both the bulged nucleotide and its flanking base pairs. The activation energy for duplex dissociation (Ea-1), however, did not always depend on the bulged nucleotide. In the case of d(TAGCGTTATAA)/d(ATCCAATATT) with one C bulge (GCG-bulge helix) and d(TAGAGTTATAA)/d(ATCCAATATT) with one A bulge (GAG-bulge helix), that had different bulged nucleotides and the same flanking base-pairs, the Ea+1 value of −13.5 kcal/mol for the GCG-bulge helix was 11.9 kcal/mol more negative than the va..." @default.
• W2044112600 created "2016-06-24" @default.
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• W2044112600 date "2000-11-04" @default.
• W2044112600 modified "2023-09-26" @default.
• W2044112600 title "Kinetic Property of Bulged Helix Formation: Analysis of Kinetic Behavior Using Nearest-Neighbor Parameters" @default.
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• W2044112600 doi "https://doi.org/10.1021/ja001779f" @default.
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