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• W2044139447 abstract "The Escherichia coli DNA‐binding protein H‐NS is known to interact specifically with the upstream region of ribosomal RNA transcription units, where it causes transcriptional repression in vitro . Here, we present results demonstrating the effect of H‐NS on rRNA transcription in vivo . rRNA synthesis rates were compared in cells that differ in the expression of functional H‐NS or FIS molecules. We could show that in the absence of H‐NS derepression of rRNA synthesis occurs at low growth rates. During the cell cycle H‐NS is responsible for the rapid shut‐off of rRNA synthesis at the end of the exponential phase. As it is known for FIS‐dependent activation, the inhibitory function of H‐NS is specific for P1, the first of the tandem rRNA promoters. The effect of H‐NS on rRNA synthesis was further assessed under stress conditions. While under osmotic upshift the reduction in rRNA synthesis is clearly H‐NS‐dependent, no such influence could be detected at cold shock. Determination of the cellular ppGpp concentrations revealed that H‐NS does not mediate its function via alterations in the synthesis of the global effector ppGpp." @default.
• W2044139447 created "2016-06-24" @default.
• W2044139447 creator A5040510726 @default.
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• W2044139447 date "1998-04-01" @default.
• W2044139447 modified "2023-09-27" @default.
• W2044139447 title "Effects of the<i>Escherichia coli</i>DNA‐binding protein H‐NS on rRNA synthesis<i>in vivo</i>" @default.
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• W2044139447 doi "https://doi.org/10.1046/j.1365-2958.1998.00829.x" @default.
• W2044139447 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9632265" @default.
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