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- W2044161738 abstract "Since oleic acid (OA) induces morphologic and cellular changes similar to those observed in human acute lung injury (ALI) and acute respiratory distress syndrome, it has become a widely used model to investigate the effects of several agents on pathogenesis of lung injury. The antioxidant and anti-inflammatory properties of N-acetylcysteine (NAC) has been documented in many lung injury models. In this study, we evaluated the role of NAC in an OA-induced lung injury model by measuring myeloperoxidase (MPO) activity, malondialdehyde (MDA) and 3-nitrotyrosine (3-NT) levels in lung tissue. Five groups labelled Sham, NAC, OA, Pre-OA–NAC and Post-OA–NAC were determined. ALI was induced by intravenous administration of OA. The pre-OA–NAC group received iv NAC 15 min before OA infusion and the post-OA–NAC group received iv NAC 2 h after OA infusion. In both of the NAC treatment groups' blood and tissue samples were collected 4 h after OA infusion, independent from the time of NAC infusion. The MPO activity, MDA and 3-NT levels in lung homogenates were found to be increased in OA group and the administration of NAC significantly reduced tissue MPO, MDA and 3-NT levels (p=0.0001). Lung histopathology was also affected by NAC in this OA-induced experimental lung injury model." @default.
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- W2044161738 date "2004-10-01" @default.
- W2044161738 modified "2023-10-15" @default.
- W2044161738 title "N-acetylcysteine inhibits peroxynitrite-mediated damage in oleic acid-induced lung injury" @default.
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- W2044161738 doi "https://doi.org/10.1016/j.pupt.2004.05.002" @default.
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