FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2044162860> ?p ?o ?g. }

• W2044162860 endingPage "946" @default.
• W2044162860 startingPage "938" @default.
• W2044162860 abstract "As one of three subunits of DNA-dependent protein kinase (DNA-PK), Ku70 protein plays an important role in repair of DNA double-strand breaks (DNA DSB). To further understand the functions of Ku70 protein and the mechanisms underlying arsenite-induced genotoxic effects, the effects of Ku70 deficiency were examined. The Ku70-deficient cell line HLFK and null vector cell line HLFC were established after recombinant plasmid of Ku70 gene antisense RNA and null pEGFP-C1 vector were transferred into human embryo lung fibroblasts (HLF) cells. Experiments were undertaken to detect DNA DSB damage by neutral single-cell gel electrophoresis assay (SCGE), chromosomal alterations by micronucleus test, and cell cycle progression by flow cytometry in HLFC and HLFK cells treated with control, 1, 2.5, 5, or 10 μM sodium arsenite for 2, 4, or 24 h, respectively. Western blot analysis results showed that Ku70 protein content in HLFK cells decreased to 38% of those in HLFC cells. The median lethal concentrations (LC50) of sodium arsenite to HLFC and HLFK cells for 24 h were 27.38 μM and 21.80 μM, respectively. Results of neutral SCGE assay showed that there were concentration-dependent increases in tail length of DNA DSB, in percent of cells with DNA DSB tails, and in severity of DNA DSB damage in HLFK and HLFC cells. The increases in these indices in HLFK cells were significantly higher than those found in HLFC cells exposed to similar amounts of metal. The ability of DNA DSB to repair in HLFK cells was less than that seen in HLFC cells. Sodium arsenite produced concentration-dependent elevation in micronuclei and abnormal nuclei formation. The Ku70-deficiency enhanced the susceptibility to chromosomal alterations induced by sodium arsenite. Low concentrations of sodium arsenite induced cell arrest at G1; however, at high concentrations of metal this G1 arrest effect disappeared. These results suggested that Ku70 protein plays an important role in repair of DNA DSB damage and for maintainance of genome stability." @default.
• W2044162860 created "2016-06-24" @default.
• W2044162860 creator A5000219612 @default.
• W2044162860 creator A5037385416 @default.
• W2044162860 creator A5055406443 @default.
• W2044162860 creator A5064023466 @default.
• W2044162860 creator A5066867449 @default.
• W2044162860 creator A5077449523 @default.
• W2044162860 date "2007-04-30" @default.
• W2044162860 modified "2023-09-26" @default.
• W2044162860 title "Arsenite-Induced Alterations in Ku70-Deficient Cells: A Model to Study Genotoxic Effects" @default.
• W2044162860 cites W1512072225 @default.
• W2044162860 cites W1515564094 @default.
• W2044162860 cites W1593571952 @default.
• W2044162860 cites W1892185927 @default.
• W2044162860 cites W1964497156 @default.
• W2044162860 cites W196512513 @default.
• W2044162860 cites W1978665041 @default.
• W2044162860 cites W1985551165 @default.
• W2044162860 cites W1988845313 @default.
• W2044162860 cites W1992688638 @default.
• W2044162860 cites W1993532042 @default.
• W2044162860 cites W1997565776 @default.
• W2044162860 cites W2012126331 @default.
• W2044162860 cites W2024877615 @default.
• W2044162860 cites W2034269086 @default.
• W2044162860 cites W2035029439 @default.
• W2044162860 cites W2036103078 @default.
• W2044162860 cites W2050173515 @default.
• W2044162860 cites W2050291003 @default.
• W2044162860 cites W2052801384 @default.
• W2044162860 cites W2056051410 @default.
• W2044162860 cites W2058247709 @default.
• W2044162860 cites W2058800908 @default.
• W2044162860 cites W2059115578 @default.
• W2044162860 cites W2061290039 @default.
• W2044162860 cites W2064293929 @default.
• W2044162860 cites W2065847176 @default.
• W2044162860 cites W2096136751 @default.
• W2044162860 cites W2096860860 @default.
• W2044162860 cites W2101864774 @default.
• W2044162860 cites W2105814326 @default.
• W2044162860 cites W2118910313 @default.
• W2044162860 cites W2126616568 @default.
• W2044162860 cites W2130728712 @default.
• W2044162860 cites W2137091485 @default.
• W2044162860 cites W2144401377 @default.
• W2044162860 cites W2159314125 @default.
• W2044162860 cites W2163476495 @default.
• W2044162860 cites W2168792406 @default.
• W2044162860 cites W2170880977 @default.
• W2044162860 doi "https://doi.org/10.1080/15287390701290253" @default.
• W2044162860 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17479409" @default.
• W2044162860 hasPublicationYear "2007" @default.
• W2044162860 type Work @default.
• W2044162860 sameAs 2044162860 @default.
• W2044162860 citedByCount "1" @default.
• W2044162860 crossrefType "journal-article" @default.
• W2044162860 hasAuthorship W2044162860A5000219612 @default.
• W2044162860 hasAuthorship W2044162860A5037385416 @default.
• W2044162860 hasAuthorship W2044162860A5055406443 @default.
• W2044162860 hasAuthorship W2044162860A5064023466 @default.
• W2044162860 hasAuthorship W2044162860A5066867449 @default.
• W2044162860 hasAuthorship W2044162860A5077449523 @default.
• W2044162860 hasConcept C116084860 @default.
• W2044162860 hasConcept C134935766 @default.
• W2044162860 hasConcept C143425029 @default.
• W2044162860 hasConcept C153911025 @default.
• W2044162860 hasConcept C178790620 @default.
• W2044162860 hasConcept C185592680 @default.
• W2044162860 hasConcept C2776305511 @default.
• W2044162860 hasConcept C2779495234 @default.
• W2044162860 hasConcept C2780919815 @default.
• W2044162860 hasConcept C502230775 @default.
• W2044162860 hasConcept C54355233 @default.
• W2044162860 hasConcept C552990157 @default.
• W2044162860 hasConcept C81885089 @default.
• W2044162860 hasConcept C86803240 @default.
• W2044162860 hasConceptScore W2044162860C116084860 @default.
• W2044162860 hasConceptScore W2044162860C134935766 @default.
• W2044162860 hasConceptScore W2044162860C143425029 @default.
• W2044162860 hasConceptScore W2044162860C153911025 @default.
• W2044162860 hasConceptScore W2044162860C178790620 @default.
• W2044162860 hasConceptScore W2044162860C185592680 @default.
• W2044162860 hasConceptScore W2044162860C2776305511 @default.
• W2044162860 hasConceptScore W2044162860C2779495234 @default.
• W2044162860 hasConceptScore W2044162860C2780919815 @default.
• W2044162860 hasConceptScore W2044162860C502230775 @default.
• W2044162860 hasConceptScore W2044162860C54355233 @default.
• W2044162860 hasConceptScore W2044162860C552990157 @default.
• W2044162860 hasConceptScore W2044162860C81885089 @default.
• W2044162860 hasConceptScore W2044162860C86803240 @default.
• W2044162860 hasIssue "11" @default.
• W2044162860 hasLocation W20441628601 @default.
• W2044162860 hasLocation W20441628602 @default.
• W2044162860 hasOpenAccess W2044162860 @default.
• W2044162860 hasPrimaryLocation W20441628601 @default.
• W2044162860 hasRelatedWork W2032791144 @default.

• next