FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W204416466> ?p ?o ?g. }

• W204416466 abstract "It is highly advantageous to be able to develop bioprocesses early on in theproduct design lifecycle, where strategic options and alternatives can beconsidered cheaply and effectively. Resources are however often in veryshort supply at this point in the process, typically with very limited quantitiesof feedstock available and with minimal access to capital equipment. As suchthere is significant advantage in being able to develop chromatographicprocesses at very small scales that will only require small quantities offeedstock and can utilise common laboratory equipment.If the height of the packed bed is not maintained during scale up then itbecomes difficult to predict chromatographic behaviour and efficiency. Thisseriously limits how small a chromatography system can be and still berepresentative of how a process scale chromatographic separation willbehave. Ultra scale-down (USD) methodologies for chromatography take adifferent approach. A small scale device, which may or may not havegeometric similarity to the process scale equipment, generates data whichwhen combined with a specific methodology and mathematical model allowsthe prediction of the large scale equivalent.The work in this thesis sought to develop a USD methodology and model toaccurately predict large scale chromatographic behaviour using very smallscale devices.The development of a model separation was required to act as a source ofrealistic experimental data for the development of a USD methodology. Thismodel separation required a suitable feedstock, a suitable USD device and asuitable sorbent on which to perform the separation.It was found that the most suitable feedstock of those tested was a FAbfragment containing periplasmic lysate produced in E. coli, due to the ease ofpre-chromatographic processing and industrial relevance. Several designs ofvery small column were investigated and it was found that PRC pre-packedcolumns (Pall Life Sciences) had superior separation characteristics and weretherefore selected as the USD device of choice. This was combined toproduce a viable separation process using MEP HyperCel presented in the PRCpre-packed column format with FAb fragment containing periplasmic lysate asa feedstock. A linear pH gradient produced a clearly resolved two peaksystem with excellent FAb fragment purity that was deemed very suitable as areference separation for the USD methodology development.The premise of the USD methodology was the deconvolution of each relevantpeak within an elution profile into its four curve coefficients, namely height,width at half height, skew and peak location. These four curve coefficientsfor each peak in the small scale chromatogram could then be individuallymodelled using a transformation function into the large scale equivalents andthen recreate a large scale chromatogram prediction from these values. Thiswas also used to predict how the chromatograms will change with respect toaltering the packed bed height and linear velocity of the loading and elutionsteps. The methodology that was developed was shown to be effective andwas typically accurate to under 5% difference normalised root mean squarewhen the predicted large scale chromatograms and real large scale data wascompared.The methodology was further validated by testing with a range of differentchromatographic systems and processes. These included changing thefeedstock by reducing the FAb fragment titre by 50%, changing thechromatographic ligand to PPA and also a multi-variate change that alteredthe ligand, the sorbent backbone and the feedstock all at once. Thetransformation functions were found not to be generic and required systemspecific alterations. However, the methodology itself was shown to be veryeffective across a wide range of chromatographic conditions and systems andas such would be a good basis for ultra-scale down development in elutionchromatography." @default.
• W204416466 created "2016-06-24" @default.
• W204416466 creator A5043235995 @default.
• W204416466 date "2011-06-28" @default.
• W204416466 modified "2023-09-26" @default.
• W204416466 title "Ultra scale-down of elution chromatography" @default.
• W204416466 cites W1498722223 @default.
• W204416466 cites W1503147674 @default.
• W204416466 cites W1517481555 @default.
• W204416466 cites W1535034058 @default.
• W204416466 cites W1540324723 @default.
• W204416466 cites W1543665493 @default.
• W204416466 cites W1552697504 @default.
• W204416466 cites W1562820395 @default.
• W204416466 cites W1569758561 @default.
• W204416466 cites W1608670310 @default.
• W204416466 cites W1931534490 @default.
• W204416466 cites W1964627739 @default.
• W204416466 cites W1965034492 @default.
• W204416466 cites W1965827193 @default.
• W204416466 cites W1966040681 @default.
• W204416466 cites W1966728669 @default.
• W204416466 cites W1969030000 @default.
• W204416466 cites W1971063580 @default.
• W204416466 cites W1971114537 @default.
• W204416466 cites W1971161994 @default.
• W204416466 cites W1971737131 @default.
• W204416466 cites W1972511084 @default.
• W204416466 cites W1972513621 @default.
• W204416466 cites W1976187304 @default.
• W204416466 cites W1979399990 @default.
• W204416466 cites W1979434921 @default.
• W204416466 cites W1982895432 @default.
• W204416466 cites W1985670478 @default.
• W204416466 cites W1987135617 @default.
• W204416466 cites W1987920176 @default.
• W204416466 cites W1989389958 @default.
• W204416466 cites W1990771970 @default.
• W204416466 cites W1995591307 @default.
• W204416466 cites W1995606157 @default.
• W204416466 cites W1996325903 @default.
• W204416466 cites W1997829807 @default.
• W204416466 cites W1999810562 @default.
• W204416466 cites W2001394491 @default.
• W204416466 cites W2001398000 @default.
• W204416466 cites W2006106749 @default.
• W204416466 cites W2006415570 @default.
• W204416466 cites W2007678582 @default.
• W204416466 cites W2008843696 @default.
• W204416466 cites W2016799976 @default.
• W204416466 cites W2021555268 @default.
• W204416466 cites W2027600997 @default.
• W204416466 cites W2028894128 @default.
• W204416466 cites W2031982324 @default.
• W204416466 cites W2032102889 @default.
• W204416466 cites W2034074754 @default.
• W204416466 cites W2035312957 @default.
• W204416466 cites W2038939053 @default.
• W204416466 cites W2039548196 @default.
• W204416466 cites W2041455774 @default.
• W204416466 cites W2042061409 @default.
• W204416466 cites W2045001999 @default.
• W204416466 cites W2045252337 @default.
• W204416466 cites W2045313823 @default.
• W204416466 cites W2045375682 @default.
• W204416466 cites W2045605530 @default.
• W204416466 cites W2045627313 @default.
• W204416466 cites W2045701458 @default.
• W204416466 cites W2047949437 @default.
• W204416466 cites W2050237533 @default.
• W204416466 cites W2051830951 @default.
• W204416466 cites W2053083611 @default.
• W204416466 cites W2055089536 @default.
• W204416466 cites W2058268622 @default.
• W204416466 cites W2059350530 @default.
• W204416466 cites W2059685185 @default.
• W204416466 cites W2061974870 @default.
• W204416466 cites W2066343625 @default.
• W204416466 cites W2066491590 @default.
• W204416466 cites W2067861278 @default.
• W204416466 cites W2068818885 @default.
• W204416466 cites W2069368645 @default.
• W204416466 cites W2071750872 @default.
• W204416466 cites W2072414250 @default.
• W204416466 cites W2072744250 @default.
• W204416466 cites W2073888623 @default.
• W204416466 cites W2073951392 @default.
• W204416466 cites W2073966218 @default.
• W204416466 cites W2074172477 @default.
• W204416466 cites W2074583603 @default.
• W204416466 cites W2075155332 @default.
• W204416466 cites W2075460275 @default.
• W204416466 cites W2082592090 @default.
• W204416466 cites W2083342131 @default.
• W204416466 cites W2084382410 @default.
• W204416466 cites W2084437389 @default.
• W204416466 cites W2085157538 @default.
• W204416466 cites W2085325777 @default.
• W204416466 cites W2086150866 @default.
• W204416466 cites W2087817580 @default.

• next