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• W2044165123 abstract "Commercial enzyme-linked immunosorbent assay (ELISA) kits for the determination of the in vitro potency of recombinant hepatitis B vaccines, which detect hepatitis B surface antigen (HBsAg), have been used frequently as an alternative for traditional in vivo potency tests. With the constant need for validation procedures, an ELISA that could be employed to determine the in vitro potency of five recombinant hepatitis B vaccines simultaneously was established using two monoclonal antibodies. The use of two monoclonal antibodies produced “in house” specific for the small envelope protein S of the hepatitis B virus (HBV) resulted in the production of a highly specific, sensitive and stable ELISA. The standard ELISA parameters used in this study, considering the HBsAg content of each recombinant hepatitis B vaccine evaluated, resulted in a standard curve that could be applied for potency evaluations of different, commercial hepatitis B vaccine lots." @default.
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• W2044165123 date "2011-03-01" @default.
• W2044165123 modified "2023-09-25" @default.
• W2044165123 title "Establishment and validation of an ELISA for the quantitation of HBsAg in recombinant hepatitis B vaccines" @default.
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• W2044165123 doi "https://doi.org/10.1016/j.jviromet.2010.12.010" @default.
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