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- W2044184449 abstract "Protein extraction is a critical step in any proteomics study. Since most fungi possess a robust cell wall, efficient isolation of total proteins has become challenge to fungal proteomics. To circumvent this bottleneck of fungal proteomics, we standardized a protocol named as Mg/CHAPS extraction by comparing with an established method of protein extraction (Tris/EDTA extraction), using 2-DE and MALDI-TOF MS. Total mycelial proteins were isolated using both protocols from Magnaporthe grisea (causal agent of rice blast disease). Six hundred forty two proteins were resolved on two 2-DE gels corresponding to mycelial proteomes isolated by Mg/CHAPS and Tris/EDTA. Mycelial proteome extracted by Mg/CHAPS showed higher number protein spots than to Tris/EDTA. Quantitative analysis of mycelial proteome, histogram and MS analyses of a protein spot suggested that Mg/CHAPS extraction is more effective than the widely used protocol i.e. Tris/EDTA." @default.
- W2044184449 created "2016-06-24" @default.
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- W2044184449 date "2010-10-01" @default.
- W2044184449 modified "2023-09-27" @default.
- W2044184449 title "Optimization of a protein extraction technique for fungal proteomics" @default.
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- W2044184449 doi "https://doi.org/10.1007/s12088-010-0072-3" @default.
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