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- W2044207631 abstract "Endothelial cell (EC) seeding is postulated as a mechanism of improving patency of small calibre vascular grafts. However, the majority of seeded cells are lost within hours following restoration of blood flow. We postulated that incubating EC in-vitro on a graft will improve adherence and resistance to the sheer stresses of pulsatile blood flow. Fibronectin-treated ePTFE (5cm × 4mm ID) seeded with Indium-111-labelled autologous canine EC (1.5 × 105 cells/cm2) were incubated for four different time periods; 90 min, 24 h, 72 h and 6 days. Incubated grafts were subjected to blood flow of 75 ml/min for 6 h, in a canine ex-vivo arteriovenous shunt circuit. EC retention during perfusion was studied by measuring gamma activity emitted by the grafts. Cell morphology of non-perfused control groups and perfused groups was compared using scanning electron microscopy (SEM). SEM of control grafts showed progressive EC spreading on the ePTFE surface for up to 72 h incubation. Gamma activity was significantly higher at 6 h perfusion in grafts incubated for 72 h (82 ± 4%) and 24 h (63 ± 6%) vs. 90 min (34 ± 13%, p < 0.05), and between grafts incubated for 72 h vs. 6 days (55 ± 7%, p < 0.05). Perfused grafts incubated for 72 h showed unaltered EC morphology on SEM, few cells remained on 90 min incubated grafts. We conclude that incubating EC on fibronectin-treated ePTFE for 72 h in-vitro after seeding improves cell retention during blood flow." @default.
- W2044207631 created "2016-06-24" @default.
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- W2044207631 date "1991-06-01" @default.
- W2044207631 modified "2023-09-23" @default.
- W2044207631 title "Increased in-vitro incubation time of endothelial cells on fibronectin-treated ePTFE increases cell retention in blood flow" @default.
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- W2044207631 doi "https://doi.org/10.1016/s0950-821x(05)80515-8" @default.
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