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- W2044282257 abstract "There is strong evidence that the acetylcholine system is responsible for the electric currents which propagate impulses in conducting tissues.<sup>1,2</sup>In the eye, high concentrations of acetylcholine have been reported in the corneal epithelium;<sup>3</sup>cholinesterase has been demonstrated in various other ocular tissues chemically<sup>4</sup>and histochemically;<sup>5,6</sup>and high choline acetylase activity has been found in the corneal epithelium.<sup>7</sup>The present study is concerned with determining the concentration and localization of cholinesterase in the cornea. <h3>Methods and Materials</h3> Hestrin's colorimetric technique for determining acetylcholine<sup>8</sup>was used for measurement of cholinesterase activity chemically, and Koelle's method for localization of the cholinesterase in the tissues.<sup>5,6,9-11</sup>Normal New Zealand albino rabbits were used throughout. <h3>A. Chemical Determination of Cholinesterase Activity.</h3> —Rabbits were anesthetized with pentobarbital (Nembutal) immediately prior to having the epithelium scraped off their corneas. The fresh epithelium was placed in a small ground glass tissue" @default.
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- W2044282257 date "1965-03-01" @default.
- W2044282257 modified "2023-09-27" @default.
- W2044282257 title "Acetylcholinesterase in the Rabbit Cornea" @default.
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- W2044282257 doi "https://doi.org/10.1001/archopht.1965.00970030372016" @default.
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