FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2044282903> ?p ?o ?g. }

• W2044282903 endingPage "61" @default.
• W2044282903 startingPage "49" @default.
• W2044282903 abstract "Cryopreservation has been extensively used in human reproductive medicine, aquaculture and conservation programmes for endangered species. However, despite the growing successes of cryopreservation, post-thaw recovery of reproductive and embryonic cells very often remains poor. Many studies have been devoted to the mechanisms of cryodamage. It is known that cryopreservation causes extensive damage to membranes; reduce the metabolic activity of cells; and disturbs the mitochondrial bioenergetical processes of cells. But few investigations on the genetic stability of cells during cryopreservation have been performed, and the role of any genetic impact cryopreservation needs to be determined. Some indirect data in the literature suggests that progress in this field might come from investigating freezing damage to mitochondrial DNA (mtDNA), nuclear DNA and other genome-related structures. In this study, zebrafish (Danio rerio) blastomeres were treated in three different ways: control suspension of blastomere cells in phosphate buffered saline; equilibration of blastomeres with 2 M dimethyl sulfoxide (Me2SO) for 1 h at room temperature and cryopreservation using Me2SO as a cryoprotectant. Mitochondrial DNA was analysed in fresh cells and after the different treatments. Two different loci of mtDNA were amplified with the help of PCR and sequenced. The sequences were analysed and nuclear base substitutions were counted for both control and treated samples. The results showed that cryopreservation significantly increased the frequency of mutations (0.78 ± 0.27% in comparison to 0.16 ± 0.25% of control), whilst 2 M Me2SO treatment did not bring a significant increase in frequency of mutations (0.24 ± 0.28%). The distributions of the mutation locations were analysed. More investigations are needed to determine whether optimisation of cryopreservation protocol is possible to reduce these adverse effects; whether such mutations interfere with overall function of the cells; whether similar changes also occur in the nuclear DNA and whether such mutations happen in other species. Meanwhile, it is important to be cautious in making judgements of the effect of cryopreservation technique in assisted reproduction. This is the first report on the effect of cryopreservation on mtDNA." @default.
• W2044282903 created "2016-06-24" @default.
• W2044282903 creator A5023169659 @default.
• W2044282903 creator A5031649400 @default.
• W2044282903 creator A5047483989 @default.
• W2044282903 creator A5056682286 @default.
• W2044282903 date "2005-02-01" @default.
• W2044282903 modified "2023-10-12" @default.
• W2044282903 title "Effect of cryopreservation on mitochondrial DNA of zebrafish (Danio rerio) blastomere cells" @default.
• W2044282903 cites W1650754258 @default.
• W2044282903 cites W1836738287 @default.
• W2044282903 cites W1967219448 @default.
• W2044282903 cites W1967974915 @default.
• W2044282903 cites W1975784350 @default.
• W2044282903 cites W1981161568 @default.
• W2044282903 cites W1988207129 @default.
• W2044282903 cites W1993149192 @default.
• W2044282903 cites W2001556707 @default.
• W2044282903 cites W2015411189 @default.
• W2044282903 cites W2018185503 @default.
• W2044282903 cites W2033608375 @default.
• W2044282903 cites W2036140173 @default.
• W2044282903 cites W2036163118 @default.
• W2044282903 cites W2038981851 @default.
• W2044282903 cites W2040175583 @default.
• W2044282903 cites W2044589186 @default.
• W2044282903 cites W2049692886 @default.
• W2044282903 cites W2074832119 @default.
• W2044282903 cites W2083815812 @default.
• W2044282903 cites W2091794450 @default.
• W2044282903 cites W2098992864 @default.
• W2044282903 cites W2114842744 @default.
• W2044282903 cites W2115130020 @default.
• W2044282903 cites W2127677621 @default.
• W2044282903 cites W2165132551 @default.
• W2044282903 doi "https://doi.org/10.1016/j.mrfmmm.2004.09.007" @default.
• W2044282903 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15680402" @default.
• W2044282903 hasPublicationYear "2005" @default.
• W2044282903 type Work @default.
• W2044282903 sameAs 2044282903 @default.
• W2044282903 citedByCount "51" @default.
• W2044282903 countsByYear W20442829032012 @default.
• W2044282903 countsByYear W20442829032013 @default.
• W2044282903 countsByYear W20442829032014 @default.
• W2044282903 countsByYear W20442829032016 @default.
• W2044282903 countsByYear W20442829032019 @default.
• W2044282903 countsByYear W20442829032020 @default.
• W2044282903 countsByYear W20442829032021 @default.
• W2044282903 countsByYear W20442829032022 @default.
• W2044282903 countsByYear W20442829032023 @default.
• W2044282903 crossrefType "journal-article" @default.
• W2044282903 hasAuthorship W2044282903A5023169659 @default.
• W2044282903 hasAuthorship W2044282903A5031649400 @default.
• W2044282903 hasAuthorship W2044282903A5047483989 @default.
• W2044282903 hasAuthorship W2044282903A5056682286 @default.
• W2044282903 hasConcept C104317684 @default.
• W2044282903 hasConcept C16685009 @default.
• W2044282903 hasConcept C179933525 @default.
• W2044282903 hasConcept C196843134 @default.
• W2044282903 hasConcept C24586158 @default.
• W2044282903 hasConcept C2776878037 @default.
• W2044282903 hasConcept C2780384893 @default.
• W2044282903 hasConcept C54355233 @default.
• W2044282903 hasConcept C59843425 @default.
• W2044282903 hasConcept C68528507 @default.
• W2044282903 hasConcept C68760520 @default.
• W2044282903 hasConcept C71924100 @default.
• W2044282903 hasConcept C86803240 @default.
• W2044282903 hasConcept C87073359 @default.
• W2044282903 hasConcept C95444343 @default.
• W2044282903 hasConceptScore W2044282903C104317684 @default.
• W2044282903 hasConceptScore W2044282903C16685009 @default.
• W2044282903 hasConceptScore W2044282903C179933525 @default.
• W2044282903 hasConceptScore W2044282903C196843134 @default.
• W2044282903 hasConceptScore W2044282903C24586158 @default.
• W2044282903 hasConceptScore W2044282903C2776878037 @default.
• W2044282903 hasConceptScore W2044282903C2780384893 @default.
• W2044282903 hasConceptScore W2044282903C54355233 @default.
• W2044282903 hasConceptScore W2044282903C59843425 @default.
• W2044282903 hasConceptScore W2044282903C68528507 @default.
• W2044282903 hasConceptScore W2044282903C68760520 @default.
• W2044282903 hasConceptScore W2044282903C71924100 @default.
• W2044282903 hasConceptScore W2044282903C86803240 @default.
• W2044282903 hasConceptScore W2044282903C87073359 @default.
• W2044282903 hasConceptScore W2044282903C95444343 @default.
• W2044282903 hasIssue "1" @default.
• W2044282903 hasLocation W20442829031 @default.
• W2044282903 hasLocation W20442829032 @default.
• W2044282903 hasOpenAccess W2044282903 @default.
• W2044282903 hasPrimaryLocation W20442829031 @default.
• W2044282903 hasRelatedWork W1997305124 @default.
• W2044282903 hasRelatedWork W2039428748 @default.
• W2044282903 hasRelatedWork W2083688289 @default.
• W2044282903 hasRelatedWork W2107422865 @default.
• W2044282903 hasRelatedWork W2123694060 @default.
• W2044282903 hasRelatedWork W2134057753 @default.
• W2044282903 hasRelatedWork W2135290162 @default.
• W2044282903 hasRelatedWork W2291928773 @default.

• next