Matches in SemOpenAlex for { <https://semopenalex.org/work/W2044287130> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W2044287130 endingPage "945" @default.
- W2044287130 startingPage "938" @default.
- W2044287130 abstract "Deuterium kinetic isotope effects (KIE) were measured, and proton inventory plots were constructed, for the rates of reactions in the photocycles of wild-type bacteriorhodopsin and several site-specific mutants. Consistent with earlier reports from many groups, very large KIEs were observed for the third (and largest) rise component for the M state and for the decay of the O state, processes both linked to proton transfers in the extracellular region. The proton inventory plots (ratio of reaction rates in mixtures of H(2)O and D(2)O to that in H(2)O vs mole fraction of D(2)O) were approximately linear for the first and second M rise components and for M decay, as well as for O decay, indicating that the rates of these reactions are limited by simple proton transfer. Uniquely, the third rise component of M (and in the D96N mutant also a fourth rise component) exhibited a strongly curved proton inventory plot, suggesting that its rate, which largely accounts for the rate of deprotonation of the retinal Schiff base, depends on a complex multiproton process. This curvature is observed also in the E194Q, E204Q, and Y57F mutants but not in the R82A mutant. From these findings, and from the locations of bound water in the extracellular region in the crystal structure of the protein [Luecke, Schobert, Richter, Cartailler, and Lanyi (1999) J. Mol. Biol. 291, 899-911], we suspect that the effects of deuterium substitution on the formation of the M state originate from cooperative rearrangements of the extensively hydrogen-bonded water molecules 401, 402, and 406 near Asp-85 and Arg-82." @default.
- W2044287130 created "2016-06-24" @default.
- W2044287130 creator A5001308102 @default.
- W2044287130 creator A5062720290 @default.
- W2044287130 creator A5079791860 @default.
- W2044287130 date "2000-01-08" @default.
- W2044287130 modified "2023-09-28" @default.
- W2044287130 title "Origins of Deuterium Kinetic Isotope Effects on the Proton Transfers of the Bacteriorhodopsin Photocycle" @default.
- W2044287130 cites W1626398746 @default.
- W2044287130 cites W1969738803 @default.
- W2044287130 cites W1991020048 @default.
- W2044287130 cites W1991421059 @default.
- W2044287130 cites W1992068738 @default.
- W2044287130 cites W1998443578 @default.
- W2044287130 cites W2000206262 @default.
- W2044287130 cites W2014375095 @default.
- W2044287130 cites W2019017134 @default.
- W2044287130 cites W2035377657 @default.
- W2044287130 cites W2039032643 @default.
- W2044287130 cites W2049551301 @default.
- W2044287130 cites W2075846905 @default.
- W2044287130 cites W2078184681 @default.
- W2044287130 cites W2088836187 @default.
- W2044287130 cites W2115357061 @default.
- W2044287130 cites W2131038313 @default.
- W2044287130 cites W2147359801 @default.
- W2044287130 cites W2154375931 @default.
- W2044287130 cites W2159059233 @default.
- W2044287130 doi "https://doi.org/10.1021/bi9921900" @default.
- W2044287130 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10653637" @default.
- W2044287130 hasPublicationYear "2000" @default.
- W2044287130 type Work @default.
- W2044287130 sameAs 2044287130 @default.
- W2044287130 citedByCount "29" @default.
- W2044287130 countsByYear W20442871302013 @default.
- W2044287130 countsByYear W20442871302015 @default.
- W2044287130 countsByYear W20442871302016 @default.
- W2044287130 countsByYear W20442871302018 @default.
- W2044287130 countsByYear W20442871302022 @default.
- W2044287130 countsByYear W20442871302023 @default.
- W2044287130 crossrefType "journal-article" @default.
- W2044287130 hasAuthorship W2044287130A5001308102 @default.
- W2044287130 hasAuthorship W2044287130A5062720290 @default.
- W2044287130 hasAuthorship W2044287130A5079791860 @default.
- W2044287130 hasConcept C118629725 @default.
- W2044287130 hasConcept C121332964 @default.
- W2044287130 hasConcept C145148216 @default.
- W2044287130 hasConcept C178790620 @default.
- W2044287130 hasConcept C184779094 @default.
- W2044287130 hasConcept C185592680 @default.
- W2044287130 hasConcept C189542057 @default.
- W2044287130 hasConcept C41625074 @default.
- W2044287130 hasConcept C54516573 @default.
- W2044287130 hasConcept C55493867 @default.
- W2044287130 hasConcept C58364064 @default.
- W2044287130 hasConcept C62520636 @default.
- W2044287130 hasConcept C69928629 @default.
- W2044287130 hasConcept C71240020 @default.
- W2044287130 hasConcept C75473681 @default.
- W2044287130 hasConcept C8010536 @default.
- W2044287130 hasConceptScore W2044287130C118629725 @default.
- W2044287130 hasConceptScore W2044287130C121332964 @default.
- W2044287130 hasConceptScore W2044287130C145148216 @default.
- W2044287130 hasConceptScore W2044287130C178790620 @default.
- W2044287130 hasConceptScore W2044287130C184779094 @default.
- W2044287130 hasConceptScore W2044287130C185592680 @default.
- W2044287130 hasConceptScore W2044287130C189542057 @default.
- W2044287130 hasConceptScore W2044287130C41625074 @default.
- W2044287130 hasConceptScore W2044287130C54516573 @default.
- W2044287130 hasConceptScore W2044287130C55493867 @default.
- W2044287130 hasConceptScore W2044287130C58364064 @default.
- W2044287130 hasConceptScore W2044287130C62520636 @default.
- W2044287130 hasConceptScore W2044287130C69928629 @default.
- W2044287130 hasConceptScore W2044287130C71240020 @default.
- W2044287130 hasConceptScore W2044287130C75473681 @default.
- W2044287130 hasConceptScore W2044287130C8010536 @default.
- W2044287130 hasIssue "5" @default.
- W2044287130 hasLocation W20442871301 @default.
- W2044287130 hasLocation W20442871302 @default.
- W2044287130 hasOpenAccess W2044287130 @default.
- W2044287130 hasPrimaryLocation W20442871301 @default.
- W2044287130 hasRelatedWork W1965104373 @default.
- W2044287130 hasRelatedWork W1968997111 @default.
- W2044287130 hasRelatedWork W1973987986 @default.
- W2044287130 hasRelatedWork W1997666207 @default.
- W2044287130 hasRelatedWork W2016684042 @default.
- W2044287130 hasRelatedWork W2044287130 @default.
- W2044287130 hasRelatedWork W2049510427 @default.
- W2044287130 hasRelatedWork W2051074761 @default.
- W2044287130 hasRelatedWork W2074855824 @default.
- W2044287130 hasRelatedWork W2110234911 @default.
- W2044287130 hasVolume "39" @default.
- W2044287130 isParatext "false" @default.
- W2044287130 isRetracted "false" @default.
- W2044287130 magId "2044287130" @default.
- W2044287130 workType "article" @default.