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- W2044294025 abstract "The phospholipid content of purified beef-heart cytochrome oxidase can be reduced to one quarter of the original value by extraction of the particle with aqueous acetone. The extracted preparation retains only about 5% of its original activity. Mitochondrial phospholipid or individual purified phospholipids restore about half the original activity and the extent of restoration is proportional to the amount of lipid taken up by the particle. Neutral lipids, bile salts, and detergents cannot duplicate the activating effect of phospholipid, but small amounts of deoxycholate can increase the effect of phospholipid. This potentiation can be correlated with the increased solubility of the enzyme complex in the presence of the bile salt. These studies suggest that phospholipid is essential for the enzymic activity of cytochrome oxidase." @default.
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- W2044294025 date "1962-10-01" @default.
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- W2044294025 title "Studies of the electron-transfer system XLVIII. Phospholipid requirements in cytochrome oxidase" @default.
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- W2044294025 doi "https://doi.org/10.1016/0006-3002(62)90732-1" @default.
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