FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2044295509> ?p ?o ?g. }

• W2044295509 abstract "We have developed a new method for the detection of nucleic acid hybridization, based on a simple latex agglutination test that can be evaluated by the unaided eye. Nucleic acid, e.g., a polymerase chain reaction (PCR) product, is denatured and incubated with polystyrene beads carrying covalently bound complementary oligonucleotide sequences. Hybridization of the nucleic acids leads to aggregation of the latex particles, thereby verifying the presence of target sequence. The test is performed at room temperature, and results are available within 10 min. As a proof of principle, the hybridization/latex agglutination assay was applied to the detection of purified PCR fragments either specific for Salmonella spp. or a synthetic sequence, and to the detection of Salmonella enterica in artificially contaminated chicken samples. A few nanograms of purified PCR fragments were detectable. In artificially contaminated chicken samples, 3 colony-forming units (cfu)/25 g were detected in one of three replicates, and 30 cfu/25 g were detected in both of two replicates when samples for PCR were taken directly from primary enrichment, demonstrating the practical applicability of this test system. Even multiplex detection might be achievable. This novel kind of assay could be useful for a range of applications where hybridization of nucleic acids, e.g., PCR fragments, is to be detected." @default.
• W2044295509 created "2016-06-24" @default.
• W2044295509 creator A5016386278 @default.
• W2044295509 creator A5057064287 @default.
• W2044295509 creator A5091810171 @default.
• W2044295509 date "2007-03-01" @default.
• W2044295509 modified "2023-09-25" @default.
• W2044295509 title "A simple nucleic acid hybridization/latex agglutination assay for the rapid detection of polymerase chain reaction amplicons" @default.
• W2044295509 cites W1595425497 @default.
• W2044295509 cites W1638863990 @default.
• W2044295509 cites W1804759123 @default.
• W2044295509 cites W1834807051 @default.
• W2044295509 cites W1902901837 @default.
• W2044295509 cites W1967680738 @default.
• W2044295509 cites W1975675191 @default.
• W2044295509 cites W1977125458 @default.
• W2044295509 cites W2008147073 @default.
• W2044295509 cites W2013080685 @default.
• W2044295509 cites W2015709186 @default.
• W2044295509 cites W2018095854 @default.
• W2044295509 cites W2019000683 @default.
• W2044295509 cites W2024563916 @default.
• W2044295509 cites W2024615553 @default.
• W2044295509 cites W2034743206 @default.
• W2044295509 cites W2036486368 @default.
• W2044295509 cites W2048842101 @default.
• W2044295509 cites W2050267364 @default.
• W2044295509 cites W2051163863 @default.
• W2044295509 cites W2057338010 @default.
• W2044295509 cites W2059102835 @default.
• W2044295509 cites W2061755273 @default.
• W2044295509 cites W2064683530 @default.
• W2044295509 cites W2064791891 @default.
• W2044295509 cites W2078820160 @default.
• W2044295509 cites W2089626596 @default.
• W2044295509 cites W2096218114 @default.
• W2044295509 cites W2097649502 @default.
• W2044295509 cites W2113311913 @default.
• W2044295509 cites W2126012074 @default.
• W2044295509 cites W2137734085 @default.
• W2044295509 cites W2143607594 @default.
• W2044295509 cites W2145321105 @default.
• W2044295509 cites W2156203236 @default.
• W2044295509 cites W2158894328 @default.
• W2044295509 cites W2167299912 @default.
• W2044295509 cites W2167903799 @default.
• W2044295509 cites W2321461976 @default.
• W2044295509 cites W2342149769 @default.
• W2044295509 cites W1971428641 @default.
• W2044295509 doi "https://doi.org/10.1016/j.mimet.2006.10.017" @default.
• W2044295509 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17173991" @default.
• W2044295509 hasPublicationYear "2007" @default.
• W2044295509 type Work @default.
• W2044295509 sameAs 2044295509 @default.
• W2044295509 citedByCount "5" @default.
• W2044295509 countsByYear W20442955092012 @default.
• W2044295509 countsByYear W20442955092014 @default.
• W2044295509 countsByYear W20442955092018 @default.
• W2044295509 crossrefType "journal-article" @default.
• W2044295509 hasAuthorship W2044295509A5016386278 @default.
• W2044295509 hasAuthorship W2044295509A5057064287 @default.
• W2044295509 hasAuthorship W2044295509A5091810171 @default.
• W2044295509 hasConcept C104317684 @default.
• W2044295509 hasConcept C129312508 @default.
• W2044295509 hasConcept C130197536 @default.
• W2044295509 hasConcept C153911025 @default.
• W2044295509 hasConcept C185592680 @default.
• W2044295509 hasConcept C24107716 @default.
• W2044295509 hasConcept C2781065037 @default.
• W2044295509 hasConcept C2781188995 @default.
• W2044295509 hasConcept C3017666073 @default.
• W2044295509 hasConcept C49105822 @default.
• W2044295509 hasConcept C523546767 @default.
• W2044295509 hasConcept C54355233 @default.
• W2044295509 hasConcept C54871578 @default.
• W2044295509 hasConcept C552990157 @default.
• W2044295509 hasConcept C55493867 @default.
• W2044295509 hasConcept C8185291 @default.
• W2044295509 hasConcept C82381507 @default.
• W2044295509 hasConcept C86803240 @default.
• W2044295509 hasConcept C90583042 @default.
• W2044295509 hasConceptScore W2044295509C104317684 @default.
• W2044295509 hasConceptScore W2044295509C129312508 @default.
• W2044295509 hasConceptScore W2044295509C130197536 @default.
• W2044295509 hasConceptScore W2044295509C153911025 @default.
• W2044295509 hasConceptScore W2044295509C185592680 @default.
• W2044295509 hasConceptScore W2044295509C24107716 @default.
• W2044295509 hasConceptScore W2044295509C2781065037 @default.
• W2044295509 hasConceptScore W2044295509C2781188995 @default.
• W2044295509 hasConceptScore W2044295509C3017666073 @default.
• W2044295509 hasConceptScore W2044295509C49105822 @default.
• W2044295509 hasConceptScore W2044295509C523546767 @default.
• W2044295509 hasConceptScore W2044295509C54355233 @default.
• W2044295509 hasConceptScore W2044295509C54871578 @default.
• W2044295509 hasConceptScore W2044295509C552990157 @default.
• W2044295509 hasConceptScore W2044295509C55493867 @default.
• W2044295509 hasConceptScore W2044295509C8185291 @default.
• W2044295509 hasConceptScore W2044295509C82381507 @default.
• W2044295509 hasConceptScore W2044295509C86803240 @default.
• W2044295509 hasConceptScore W2044295509C90583042 @default.

• next