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- W2044306808 abstract "Studies were conducted to determine if Phomopsis viticola can infect grape berries directly and cause latent infections, or if fruit infection occurs via mycelial invasion from infected rachis tissues. Examination of inoculated immature berries using scanning electron microscopy showed that alpha spores of P. viticola germinated on the surface of the berry, produced appressoria, and appeared to penetrate the cuticle. Intact clusters of immature berries on ‘Seyval’ grape vines were inoculated with a spore suspension of P. viticola. Clusters were harvested after various periods (12, 18, 24 and 30 hr) of continuous wetness at 20°C. In a separate study, inoculated berries were incubated for 24 hr at 20°C then moved to a greenhouse for incubation periods of 1, 2, and 3 weeks. Immediately after drying (first study) or after the incubation period (second study) berries were separated from rachises and surface disinfested. Each rachis was divided into 10 sections and was also surface disinfested. Berries and rachis sections were then immersed in a 1:32 dilution of 37% paraquat dichloride to kill plant tissues and induce growth of the fungus. Abundant mycelia and pycnidia of P. viticola formed on inoculated berries and rachis sections for all wetness periods and incubation periods. Results demonstrate that direct infection of berries can result in latent infections on green fruit. Whereas the fungus may invade berries via infected rachis tissues, rachis infection is not required for Phomopsis fruit rot development. Accepted for publication 16 June 2002. Published 2 July 2002." @default.
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- W2044306808 date "2002-01-01" @default.
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- W2044306808 title "Infection of Grape Berry and Rachis Tissues by Phomopsis viticola" @default.
- W2044306808 doi "https://doi.org/10.1094/php-2002-0702-01-rs" @default.
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