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- W2044347175 abstract "Abstract The thin-layer chromatographic mobilities of pheophorbide a (Phor-a) and six metal-Phor-a complexes were measured on an octadecyl-bonded silica gel (C 18 silica) thin layer with various polar developing solvents, such as methanol, ethanol, acetonitrile and their mixtures with an aqueous solution containing an electrolyte such as sodium chloride. The mobility of the metal complex decreases in the following order of complexed metal ions: Co III > Fe III > Zn II > Phor-a > Ni II ≈ Pd II > Cu II , although the Fe III complex shows an exceptionally smaller mobility than the Zn II complex when pure acetonitrile or the water-acetonitrile containing sodium chloride is used as the developing solvent. The mobility of a metal-Phor-a complex is generally smaller than that of the pheophorbide b complex of the same metal. A successful reversed-phase high-performance liquid chromatographic separation was achieved for a mixture of Phor-a and its Fe III , Co III , Cu II and Zn II complexes by using ethanol-phosphate buffer (pH 3) (85:15, v/v) as the mobile phase." @default.
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- W2044347175 date "1988-01-01" @default.
- W2044347175 modified "2023-09-27" @default.
- W2044347175 title "Reversed-phase high-performance thin-layer chromatography and column liquid chromatography of metal complexes of pheophorbidea" @default.
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- W2044347175 doi "https://doi.org/10.1016/s0021-9673(01)82058-6" @default.
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