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• W2044354987 abstract "The goal of metabolic flux analysis (MFA) is the accurate estimation of intracellular fluxes in metabolic networks. Here, we introduce a new method for MFA based on tandem mass spectrometry (MS) and stable-isotope tracer experiments. We demonstrate that tandem MS provides more labeling information than can be obtained from traditional full scan MS analysis and allows estimation of fluxes with better precision. We present a modeling framework that takes full advantage of the additional labeling information obtained from tandem MS for MFA. We show that tandem MS data can be computed for any network model, any compound and any tandem MS fragmentation using linear mapping of isotopomers. The inherent advantages of tandem MS were illustrated in two network models using simulated and literature data. Application of tandem MS increased the observability of the models and improved the precision of estimated fluxes by 2- to 5-fold compared to traditional MS analysis." @default.
• W2044354987 created "2016-06-24" @default.
• W2044354987 creator A5046544077 @default.
• W2044354987 creator A5060360737 @default.
• W2044354987 date "2011-03-01" @default.
• W2044354987 modified "2023-10-16" @default.
• W2044354987 title "Tandem mass spectrometry: A novel approach for metabolic flux analysis" @default.
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• W2044354987 doi "https://doi.org/10.1016/j.ymben.2010.11.006" @default.
• W2044354987 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21134484" @default.
• W2044354987 hasPublicationYear "2011" @default.
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