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- W2044361502 abstract "Both transient and stable dimers of metallothionein have been characterized, based on earlier studies using NMR, circular dichroism and size-exclusion chromatography. Here additional characterization is provided by nanospray mass spectrometry. Rapid redistribution of metal ions between monomeric Cd7- and Zn7-metallothionein 2a is monitored by nanospray. An experiment in which theses two forms of the monomeric protein are separated by a dialysis membrane, which will pass metal ions but not proteins, confirms that a transient dimer must form for metal ions to be redistributed. On the other hand, size-exclusion chromatography of reconstituted Zn7- or Cd7-metallothionein revealed the presence of monomeric and dimeric species. These dimers do not equilibrate readily to form monomers and they are shown to be covalent." @default.
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- W2044361502 date "2002-01-01" @default.
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- W2044361502 title "Metallothionein dimers studied by nano-spray mass spectrometry" @default.
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- W2044361502 doi "https://doi.org/10.1016/s0162-0134(01)00375-0" @default.
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