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• W2044367003 endingPage "110" @default.
• W2044367003 startingPage "97" @default.
• W2044367003 abstract "Regulators of G protein signaling (RGS) proteins bind to the alpha subunits of certain heterotrimeric G proteins and greatly enhance their rate of GTP hydrolysis, thereby determining the time course of interactions among Galpha, Gbetagamma, and their effectors. Voltage-gated N-type Ca channels mediate neurosecretion, and these Ca channels are powerfully inhibited by G proteins. To determine whether RGS proteins could influence Ca channel function, we recorded the activity of N-type Ca channels coexpressed in human embryonic kidney (HEK293) cells with G protein-coupled muscarinic (m2) receptors and various RGS proteins. Coexpression of full-length RGS3T, RGS3, or RGS8 significantly attenuated the magnitude of receptor-mediated Ca channel inhibition. In control cells expressing alpha1B, alpha2, and beta3 Ca channel subunits and m2 receptors, carbachol (1 microM) inhibited whole-cell currents by approximately 80% compared with only approximately 55% inhibition in cells also expressing exogenous RGS protein. A similar effect was produced by expression of the conserved core domain of RGS8. The attenuation of Ca current inhibition resulted primarily from a shift in the steady state dose-response relationship to higher agonist concentrations, with the EC50 for carbachol inhibition being approximately 18 nM in control cells vs. approximately 150 nM in RGS-expressing cells. The kinetics of Ca channel inhibition were also modified by RGS. Thus, in cells expressing RGS3T, the decay of prepulse facilitation was slower, and recovery of Ca channels from inhibition after agonist removal was faster than in control cells. The effects of RGS proteins on Ca channel modulation can be explained by their ability to act as GTPase-accelerating proteins for some Galpha subunits. These results suggest that RGS proteins may play important roles in shaping the magnitude and kinetics of physiological events, such as neurosecretion, that involve G protein-modulated Ca channels." @default.
• W2044367003 created "2016-06-24" @default.
• W2044367003 creator A5014195426 @default.
• W2044367003 creator A5040916542 @default.
• W2044367003 creator A5080153002 @default.
• W2044367003 creator A5080784169 @default.
• W2044367003 date "1999-01-01" @default.
• W2044367003 modified "2023-10-18" @default.
• W2044367003 title "Regulators of G Protein Signaling Attenuate the G Protein–mediated Inhibition of N-Type Ca Channels" @default.
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• W2044367003 doi "https://doi.org/10.1085/jgp.113.1.97" @default.
• W2044367003 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2222986" @default.
• W2044367003 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9874691" @default.
• W2044367003 hasPublicationYear "1999" @default.
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