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- W2044395170 abstract "Abstract Background & Aims Using high‐density human recombinant protein microarrays, we identified two potential biomarkers, kelch‐like 12 ( KLHL 12) and hexokinase‐1 ( HK 1), in primary biliary cirrhosis ( PBC ). The objective of this study was to determine the diagnostic value of anti‐ KLHL 12/ HK 1 autoantibodies in PBC . Initial discovery used sera from 22 patients with PBC and 62 non‐ PBC controls. KLHL 12 and HK 1 proteins were then analysed for immunoglobulin reactivity by immunoblot and enzyme‐linked immunosorbent assay ( ELISA ) in two independent cohorts of PBC and disease/healthy control patients. Methods Serum samples from 100 patients with PBC and 165 non‐ PBC disease controls were analysed by immunoblot and samples from 366 patients with PBC , 174 disease controls, and 80 healthy donors were tested by ELISA . Results Anti‐ KLHL 12 and anti‐ HK 1 antibodies were each detected more frequently in PBC compared with non‐ PBC disease controls ( P < 0.001). Not only are both markers highly specific for PBC (≥95%) but they also yielded higher sensitivity than anti‐gp210 and anti‐sp100 antibodies. Combining anti‐ HK 1 and anti‐ KLHL 12 with available markers ( MIT 3, gp210 and sp100), increased the diagnostic sensitivity for PBC . Most importantly, anti‐ KLHL 12 and anti‐ HK 1 antibodies were present in 10–35% of anti‐mitochondrial antibody ( AMA )‐negative PBC patients and adding these two biomarkers to conventional PBC assays dramatically improved the serological sensitivity in AMA ‐negative PBC from 55% to 75% in immunoblot and 48.3% to 68.5% in ELISA . Conclusions The addition of tests for highly specific anti‐ KLHL 12 and anti‐ HK 1 antibodies to AMA and ANA serological assays significantly improves efficacy in the clinical detection and diagnosis of PBC , especially for AMA ‐negative subjects." @default.
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- W2044395170 date "2014-10-10" @default.
- W2044395170 modified "2023-10-15" @default.
- W2044395170 title "Anti-kelch-like 12 and anti-hexokinase 1: novel autoantibodies in primary biliary cirrhosis" @default.
- W2044395170 cites W124553404 @default.
- W2044395170 cites W1479958405 @default.
- W2044395170 cites W1556982637 @default.
- W2044395170 cites W1964240493 @default.
- W2044395170 cites W1964564102 @default.
- W2044395170 cites W1965773410 @default.
- W2044395170 cites W1970824804 @default.
- W2044395170 cites W1974366822 @default.
- W2044395170 cites W1984065159 @default.
- W2044395170 cites W1984613038 @default.
- W2044395170 cites W1990689762 @default.
- W2044395170 cites W1991166786 @default.
- W2044395170 cites W1994082232 @default.
- W2044395170 cites W1998963240 @default.
- W2044395170 cites W1999771079 @default.
- W2044395170 cites W1999813341 @default.
- W2044395170 cites W2001655471 @default.
- W2044395170 cites W2009911573 @default.
- W2044395170 cites W2012523238 @default.
- W2044395170 cites W2022251904 @default.
- W2044395170 cites W2023831900 @default.
- W2044395170 cites W2024346598 @default.
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- W2044395170 cites W2050561023 @default.
- W2044395170 cites W2054645758 @default.
- W2044395170 cites W2057363338 @default.
- W2044395170 cites W2062808435 @default.
- W2044395170 cites W2064872372 @default.
- W2044395170 cites W2065254804 @default.
- W2044395170 cites W2065714966 @default.
- W2044395170 cites W2069458216 @default.
- W2044395170 cites W2072007142 @default.
- W2044395170 cites W2084880276 @default.
- W2044395170 cites W2086002849 @default.
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- W2044395170 cites W2100262552 @default.
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- W2044395170 cites W2143657331 @default.
- W2044395170 cites W2144756716 @default.
- W2044395170 cites W2152758419 @default.
- W2044395170 cites W2159434940 @default.
- W2044395170 cites W2169404505 @default.
- W2044395170 cites W2735553444 @default.
- W2044395170 cites W4233751197 @default.
- W2044395170 cites W4292540530 @default.
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- W2044395170 doi "https://doi.org/10.1111/liv.12690" @default.
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