Matches in SemOpenAlex for { <https://semopenalex.org/work/W2044466382> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W2044466382 endingPage "2242" @default.
- W2044466382 startingPage "2233" @default.
- W2044466382 abstract "Site-directed mutagenesis experiments on all the conserved residues of Phe and Tyr in all the known squalene-hopene cyclases (SHCs) were carried out to identify the active site residues of thermophilic Alicyclobacillus acidocaldarius SHC. The following functions are proposed on the basis of kinetic data and trapping of the prematurely cyclized products: (1) The Y495 residue probably amplifies the D376 acidity, which is assumed to work as a proton donor for initiating the polycyclization cascade, but its role is moderate. (2) Y609 possibly assists the function of F365, which has previously been assigned to exclusively stabilize the C-8 carbocation intermediate through cation-π interaction. The Y609A mutant produced a partially cyclized bicyclic triterpene. (3) Y612 works to stabilize both the C10 and C8 carbocations, this being verified by the finding that mono- and bicyclic products were formed with the Y612A mutant. (4) F129 was first identified to play a crucial role in catalysis. (5) The three redsidues, Y372, Y474 and Y540, are responsible for reinforcing the protein structure against thermal denaturation, Y474 being located inside QW motif 3." @default.
- W2044466382 created "2016-06-24" @default.
- W2044466382 creator A5022193625 @default.
- W2044466382 creator A5056901462 @default.
- W2044466382 date "2001-01-01" @default.
- W2044466382 modified "2023-10-14" @default.
- W2044466382 title "Catalytic Function of the Residues of Phenylalanine and Tyrosine Conserved in Squalene-Hopene Cyclases" @default.
- W2044466382 cites W1972099761 @default.
- W2044466382 cites W1974048534 @default.
- W2044466382 cites W1982132238 @default.
- W2044466382 cites W1991316569 @default.
- W2044466382 cites W2000468537 @default.
- W2044466382 cites W2001643810 @default.
- W2044466382 cites W2008376811 @default.
- W2044466382 cites W2011187123 @default.
- W2044466382 cites W2017950861 @default.
- W2044466382 cites W2023225982 @default.
- W2044466382 cites W2025507174 @default.
- W2044466382 cites W2040674608 @default.
- W2044466382 cites W2042925473 @default.
- W2044466382 cites W2046373460 @default.
- W2044466382 cites W2048506568 @default.
- W2044466382 cites W2050293242 @default.
- W2044466382 cites W2054897769 @default.
- W2044466382 cites W2058154102 @default.
- W2044466382 cites W2063075408 @default.
- W2044466382 cites W2070779989 @default.
- W2044466382 cites W2079413454 @default.
- W2044466382 cites W2079604571 @default.
- W2044466382 cites W2117761284 @default.
- W2044466382 cites W2134266820 @default.
- W2044466382 cites W4233804102 @default.
- W2044466382 cites W4248499031 @default.
- W2044466382 doi "https://doi.org/10.1271/bbb.65.2233" @default.
- W2044466382 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11758915" @default.
- W2044466382 hasPublicationYear "2001" @default.
- W2044466382 type Work @default.
- W2044466382 sameAs 2044466382 @default.
- W2044466382 citedByCount "38" @default.
- W2044466382 countsByYear W20444663822012 @default.
- W2044466382 countsByYear W20444663822013 @default.
- W2044466382 countsByYear W20444663822014 @default.
- W2044466382 countsByYear W20444663822015 @default.
- W2044466382 countsByYear W20444663822016 @default.
- W2044466382 countsByYear W20444663822017 @default.
- W2044466382 countsByYear W20444663822018 @default.
- W2044466382 countsByYear W20444663822023 @default.
- W2044466382 crossrefType "journal-article" @default.
- W2044466382 hasAuthorship W2044466382A5022193625 @default.
- W2044466382 hasAuthorship W2044466382A5056901462 @default.
- W2044466382 hasBestOaLocation W20444663821 @default.
- W2044466382 hasConcept C109057382 @default.
- W2044466382 hasConcept C155647269 @default.
- W2044466382 hasConcept C161790260 @default.
- W2044466382 hasConcept C185592680 @default.
- W2044466382 hasConcept C24961977 @default.
- W2044466382 hasConcept C2776703722 @default.
- W2044466382 hasConcept C2777431362 @default.
- W2044466382 hasConcept C2781338088 @default.
- W2044466382 hasConcept C41183919 @default.
- W2044466382 hasConcept C515207424 @default.
- W2044466382 hasConcept C55493867 @default.
- W2044466382 hasConcept C71240020 @default.
- W2044466382 hasConceptScore W2044466382C109057382 @default.
- W2044466382 hasConceptScore W2044466382C155647269 @default.
- W2044466382 hasConceptScore W2044466382C161790260 @default.
- W2044466382 hasConceptScore W2044466382C185592680 @default.
- W2044466382 hasConceptScore W2044466382C24961977 @default.
- W2044466382 hasConceptScore W2044466382C2776703722 @default.
- W2044466382 hasConceptScore W2044466382C2777431362 @default.
- W2044466382 hasConceptScore W2044466382C2781338088 @default.
- W2044466382 hasConceptScore W2044466382C41183919 @default.
- W2044466382 hasConceptScore W2044466382C515207424 @default.
- W2044466382 hasConceptScore W2044466382C55493867 @default.
- W2044466382 hasConceptScore W2044466382C71240020 @default.
- W2044466382 hasIssue "10" @default.
- W2044466382 hasLocation W20444663821 @default.
- W2044466382 hasLocation W20444663822 @default.
- W2044466382 hasOpenAccess W2044466382 @default.
- W2044466382 hasPrimaryLocation W20444663821 @default.
- W2044466382 hasRelatedWork W1999940613 @default.
- W2044466382 hasRelatedWork W2003428175 @default.
- W2044466382 hasRelatedWork W2027634917 @default.
- W2044466382 hasRelatedWork W2032536428 @default.
- W2044466382 hasRelatedWork W2070685113 @default.
- W2044466382 hasRelatedWork W2951468791 @default.
- W2044466382 hasRelatedWork W3088499326 @default.
- W2044466382 hasRelatedWork W4230675013 @default.
- W2044466382 hasRelatedWork W578725727 @default.
- W2044466382 hasRelatedWork W2146906336 @default.
- W2044466382 hasVolume "65" @default.
- W2044466382 isParatext "false" @default.
- W2044466382 isRetracted "false" @default.
- W2044466382 magId "2044466382" @default.
- W2044466382 workType "article" @default.