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- W2044477551 abstract "Abstract Erythrocyte NADH-and NADPH-diaphorases (so-called methemoglobin reductases) have been shown to be different enzymes. A deficiency of NADH-diaphorase activity results in congenital methemoglobinemia ( Gibson, 1948 ; Scott and Griffith, 1959 ; Jaffe, 1966b ). Further understanding of this abnormality has been hampered by the fact that no satisfactory method for staining diaphorases after gel electrophoresis has been devised previously. We now present a method for staining both diaphorases after starch gel electrophoresis and describe the results obtained with normal and NADH-diaphorase-deficient red cells. In one subject with congenital methemoglobinemia an electrophoretic variant (California) was found; in two other cases, this abnormality was not found and it is therefore believed that deficiency of NADH-diaphorase is genetically and biochemically heterogeneous." @default.
- W2044477551 created "2016-06-24" @default.
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- W2044477551 date "1967-11-01" @default.
- W2044477551 modified "2023-10-18" @default.
- W2044477551 title "Electrophoresis of red cell NADH- and NADPH-diaphorases in normal subjects and patients with congenital methemoglobinemia" @default.
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- W2044477551 doi "https://doi.org/10.1016/0006-291x(67)90529-3" @default.
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