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- W2044500921 abstract "A sensitive and specific method for the determination of N-[1(R){[1,2-dihydro-1-methylsulfonylspiro(3H-indole-3,4′-piperidin)-1′-yl]carbonyl}-2-(phenylmethoxy)ethyl]-2-amino-2-methylpropanamide (MK-677, I), a growth hormone secretagogue, has been developed. The method is based on high-performance liquid chromatography (HPLC) with tandem mass spectrometric (MS–MS) detection. The analyte and internal standard (II) were isolated from the basified plasma using a liquid–liquid extraction with methyl-tert.-butyl ether (MTBE). The organic extract was evaporated to dryness, the residue was reconstituted in mobile phase and injected into the HPLC system. The MS–MS detection was performed on a PE Sciex API III Plus tandem mass spectrometer using a heated nebulizer interface. Multiple reaction monitoring of parent→product ion combinations at m/z 529→267 and 527→267 was used to quantify I and II, respectively. The assay was validated in human plasma in the concentration range of 0.1 to 100 ng/ml, and the limit of quantification (LOQ) was 0.1 ng/ml. The precision of the assay, as expressed as coefficients of variation (C.V.,%) was less than 7% at all concentrations within the standard curve range, with adequate assay specificity and accuracy. The HPLC–MS–MS method provided sufficient sensitivity to completely map the pharmacokinetic time-course following a single 5-mg oral dose of I." @default.
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- W2044500921 date "1997-05-01" @default.
- W2044500921 modified "2023-10-07" @default.
- W2044500921 title "Determination of a novel growth hormone secretagogue (MK-677) in human plasma at picogram levels by liquid chromatography with atmospheric pressure chemical ionization tandem mass spectrometry" @default.
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- W2044500921 doi "https://doi.org/10.1016/s0378-4347(97)00035-2" @default.
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