FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2044508439> ?p ?o ?g. }

• W2044508439 endingPage "e56772" @default.
• W2044508439 startingPage "e56772" @default.
• W2044508439 abstract "Quinoxaline-2-carboxylic acid (QXC) and 3-hydroxyquinaldic acid (HQA) feature in quinomycin family and confer anticancer activity. In light of the significant potency against cancer, the biosynthetic gene clusters have been reported from many different Streptomyces strains, and the biosynthetic pathway were proposed mainly based on the in vivo feeding experiment with isotope labeled putative intermediates. Herein we report another gene cluster from Streptomyces griseovariabilis subsp. bandungensis subsp. nov responsible for the biosynthesis of echinomycin (a member of quinomycin family, also named quinomycin A) and presented in vitro evidence to corroborate the previous hypothesis on QXC biosynthesis, showing that only with the assistance of a MbtH-like protein Qui5, did the didomain NRPS protein (Qui18) perform the loading of a L-tryptophan onto its own PCP domain. Particularly, it was found that Qui5 and Qui18 subunits form a functional tetramer through size exclusion chromatography. The subsequent hydroxylation on β-carbon of the loaded L-tryptophan proved in vitro to be completed by cytochrome P450-dependent hydroxylase Qui15. Importantly, only the Qui18 loaded L-tryptophan can be hydroxylated by Qui15 and the enzyme was inactive on free L-tryptophan. Additionally, the chemically synthesized (2S,3S) β-hydroxytryptophan was detected to be converted by the tryptophan 2,3-dioxygenase Qui17 through LC-MS, which enriched our previous knowledge that tryptophan 2,3-dioxygenase nearly exclusively acted on L-tryptophan and 6-fluoro-tryptophan." @default.
• W2044508439 created "2016-06-24" @default.
• W2044508439 creator A5005455567 @default.
• W2044508439 creator A5022201090 @default.
• W2044508439 creator A5027084532 @default.
• W2044508439 creator A5034855502 @default.
• W2044508439 creator A5043763813 @default.
• W2044508439 creator A5058369517 @default.
• W2044508439 creator A5062418513 @default.
• W2044508439 creator A5080351759 @default.
• W2044508439 creator A5089035316 @default.
• W2044508439 date "2013-02-21" @default.
• W2044508439 modified "2023-10-16" @default.
• W2044508439 title "In Vitro Characterization of Echinomycin Biosynthesis: Formation and Hydroxylation of L-Tryptophanyl-S-Enzyme and Oxidation of (2S,3S) β-Hydroxytryptophan" @default.
• W2044508439 cites W1975132961 @default.
• W2044508439 cites W1987358726 @default.
• W2044508439 cites W1991472040 @default.
• W2044508439 cites W1992240758 @default.
• W2044508439 cites W1994724335 @default.
• W2044508439 cites W1996467013 @default.
• W2044508439 cites W2000322651 @default.
• W2044508439 cites W2004263583 @default.
• W2044508439 cites W2006326877 @default.
• W2044508439 cites W2008815057 @default.
• W2044508439 cites W2020179990 @default.
• W2044508439 cites W2023950913 @default.
• W2044508439 cites W2046814152 @default.
• W2044508439 cites W2052430670 @default.
• W2044508439 cites W2064612440 @default.
• W2044508439 cites W2085950639 @default.
• W2044508439 cites W2091900282 @default.
• W2044508439 cites W2120508985 @default.
• W2044508439 cites W2135255584 @default.
• W2044508439 cites W2145047933 @default.
• W2044508439 cites W2149901522 @default.
• W2044508439 cites W2158714788 @default.
• W2044508439 cites W2159673356 @default.
• W2044508439 cites W2171920440 @default.
• W2044508439 cites W218459934 @default.
• W2044508439 doi "https://doi.org/10.1371/journal.pone.0056772" @default.
• W2044508439 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3578932" @default.
• W2044508439 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23437232" @default.
• W2044508439 hasPublicationYear "2013" @default.
• W2044508439 type Work @default.
• W2044508439 sameAs 2044508439 @default.
• W2044508439 citedByCount "35" @default.
• W2044508439 countsByYear W20445084392013 @default.
• W2044508439 countsByYear W20445084392014 @default.
• W2044508439 countsByYear W20445084392015 @default.
• W2044508439 countsByYear W20445084392016 @default.
• W2044508439 countsByYear W20445084392017 @default.
• W2044508439 countsByYear W20445084392018 @default.
• W2044508439 countsByYear W20445084392019 @default.
• W2044508439 countsByYear W20445084392020 @default.
• W2044508439 countsByYear W20445084392022 @default.
• W2044508439 countsByYear W20445084392023 @default.
• W2044508439 crossrefType "journal-article" @default.
• W2044508439 hasAuthorship W2044508439A5005455567 @default.
• W2044508439 hasAuthorship W2044508439A5022201090 @default.
• W2044508439 hasAuthorship W2044508439A5027084532 @default.
• W2044508439 hasAuthorship W2044508439A5034855502 @default.
• W2044508439 hasAuthorship W2044508439A5043763813 @default.
• W2044508439 hasAuthorship W2044508439A5058369517 @default.
• W2044508439 hasAuthorship W2044508439A5062418513 @default.
• W2044508439 hasAuthorship W2044508439A5080351759 @default.
• W2044508439 hasAuthorship W2044508439A5089035316 @default.
• W2044508439 hasBestOaLocation W20445084391 @default.
• W2044508439 hasConcept C104317684 @default.
• W2044508439 hasConcept C170493617 @default.
• W2044508439 hasConcept C181199279 @default.
• W2044508439 hasConcept C185592680 @default.
• W2044508439 hasConcept C2775864247 @default.
• W2044508439 hasConcept C2775951788 @default.
• W2044508439 hasConcept C2776706248 @default.
• W2044508439 hasConcept C2776834422 @default.
• W2044508439 hasConcept C2781109383 @default.
• W2044508439 hasConcept C36857842 @default.
• W2044508439 hasConcept C37000724 @default.
• W2044508439 hasConcept C515207424 @default.
• W2044508439 hasConcept C526171541 @default.
• W2044508439 hasConcept C553450214 @default.
• W2044508439 hasConcept C55493867 @default.
• W2044508439 hasConcept C71240020 @default.
• W2044508439 hasConcept C86803240 @default.
• W2044508439 hasConceptScore W2044508439C104317684 @default.
• W2044508439 hasConceptScore W2044508439C170493617 @default.
• W2044508439 hasConceptScore W2044508439C181199279 @default.
• W2044508439 hasConceptScore W2044508439C185592680 @default.
• W2044508439 hasConceptScore W2044508439C2775864247 @default.
• W2044508439 hasConceptScore W2044508439C2775951788 @default.
• W2044508439 hasConceptScore W2044508439C2776706248 @default.
• W2044508439 hasConceptScore W2044508439C2776834422 @default.
• W2044508439 hasConceptScore W2044508439C2781109383 @default.
• W2044508439 hasConceptScore W2044508439C36857842 @default.
• W2044508439 hasConceptScore W2044508439C37000724 @default.
• W2044508439 hasConceptScore W2044508439C515207424 @default.
• W2044508439 hasConceptScore W2044508439C526171541 @default.
• W2044508439 hasConceptScore W2044508439C553450214 @default.

• next