Matches in SemOpenAlex for { <https://semopenalex.org/work/W2044587411> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W2044587411 endingPage "52" @default.
- W2044587411 startingPage "49" @default.
- W2044587411 abstract "An efficient Escherichia coli system for the production of a variant form of high-mobility group-2a protein (HMG 2a), having the additional 5 amino acid residues (Ala-Pro-Thr-Leu-Glu) at the NH2-terminal, has been constructed. cDNA encoding HMG 2a was ligated with the Omp A signal peptide sequence and was inserted into an inducible bacterial expression vector pSH-L. After the plasmid introduced into E. coli was expressed by temperature shift, the recombinant product was purified by trichloacetic acid precipitation followed by Bio-Rex 70 column chromatography. The purified product showed the expected NH2-terminal sequence and the superhelical activity of circular DNA similar to the authentic HMG 2a isolated from chick liver." @default.
- W2044587411 created "2016-06-24" @default.
- W2044587411 creator A5001620097 @default.
- W2044587411 creator A5021959948 @default.
- W2044587411 creator A5031486093 @default.
- W2044587411 creator A5065115046 @default.
- W2044587411 creator A5072304907 @default.
- W2044587411 creator A5077823791 @default.
- W2044587411 creator A5088813579 @default.
- W2044587411 date "1995-06-19" @default.
- W2044587411 modified "2023-10-18" @default.
- W2044587411 title "Production of functional chick liver HMG 2a protein inEscherichia coli" @default.
- W2044587411 cites W1213045642 @default.
- W2044587411 cites W1523642307 @default.
- W2044587411 cites W1545780476 @default.
- W2044587411 cites W1547438216 @default.
- W2044587411 cites W1590373873 @default.
- W2044587411 cites W1590983439 @default.
- W2044587411 cites W1976530300 @default.
- W2044587411 cites W2002278814 @default.
- W2044587411 cites W2010919196 @default.
- W2044587411 cites W2019894624 @default.
- W2044587411 cites W2021239913 @default.
- W2044587411 cites W2029462714 @default.
- W2044587411 cites W2071894222 @default.
- W2044587411 cites W2083055584 @default.
- W2044587411 cites W2088889571 @default.
- W2044587411 cites W2100837269 @default.
- W2044587411 cites W2285663888 @default.
- W2044587411 cites W84732353 @default.
- W2044587411 cites W2087872182 @default.
- W2044587411 doi "https://doi.org/10.1016/0014-5793(95)00502-z" @default.
- W2044587411 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7601282" @default.
- W2044587411 hasPublicationYear "1995" @default.
- W2044587411 type Work @default.
- W2044587411 sameAs 2044587411 @default.
- W2044587411 citedByCount "2" @default.
- W2044587411 crossrefType "journal-article" @default.
- W2044587411 hasAuthorship W2044587411A5001620097 @default.
- W2044587411 hasAuthorship W2044587411A5021959948 @default.
- W2044587411 hasAuthorship W2044587411A5031486093 @default.
- W2044587411 hasAuthorship W2044587411A5065115046 @default.
- W2044587411 hasAuthorship W2044587411A5072304907 @default.
- W2044587411 hasAuthorship W2044587411A5077823791 @default.
- W2044587411 hasAuthorship W2044587411A5088813579 @default.
- W2044587411 hasConcept C104317684 @default.
- W2044587411 hasConcept C10858879 @default.
- W2044587411 hasConcept C153911025 @default.
- W2044587411 hasConcept C167625842 @default.
- W2044587411 hasConcept C187882448 @default.
- W2044587411 hasConcept C22744801 @default.
- W2044587411 hasConcept C33161422 @default.
- W2044587411 hasConcept C40767141 @default.
- W2044587411 hasConcept C515207424 @default.
- W2044587411 hasConcept C547475151 @default.
- W2044587411 hasConcept C552990157 @default.
- W2044587411 hasConcept C55493867 @default.
- W2044587411 hasConcept C86803240 @default.
- W2044587411 hasConceptScore W2044587411C104317684 @default.
- W2044587411 hasConceptScore W2044587411C10858879 @default.
- W2044587411 hasConceptScore W2044587411C153911025 @default.
- W2044587411 hasConceptScore W2044587411C167625842 @default.
- W2044587411 hasConceptScore W2044587411C187882448 @default.
- W2044587411 hasConceptScore W2044587411C22744801 @default.
- W2044587411 hasConceptScore W2044587411C33161422 @default.
- W2044587411 hasConceptScore W2044587411C40767141 @default.
- W2044587411 hasConceptScore W2044587411C515207424 @default.
- W2044587411 hasConceptScore W2044587411C547475151 @default.
- W2044587411 hasConceptScore W2044587411C552990157 @default.
- W2044587411 hasConceptScore W2044587411C55493867 @default.
- W2044587411 hasConceptScore W2044587411C86803240 @default.
- W2044587411 hasIssue "1" @default.
- W2044587411 hasLocation W20445874111 @default.
- W2044587411 hasLocation W20445874112 @default.
- W2044587411 hasOpenAccess W2044587411 @default.
- W2044587411 hasPrimaryLocation W20445874111 @default.
- W2044587411 hasRelatedWork W1786536704 @default.
- W2044587411 hasRelatedWork W20164601 @default.
- W2044587411 hasRelatedWork W2054826021 @default.
- W2044587411 hasRelatedWork W2171305187 @default.
- W2044587411 hasRelatedWork W2350328043 @default.
- W2044587411 hasRelatedWork W2356749314 @default.
- W2044587411 hasRelatedWork W2362399605 @default.
- W2044587411 hasRelatedWork W2371207394 @default.
- W2044587411 hasRelatedWork W2382744586 @default.
- W2044587411 hasRelatedWork W2915002842 @default.
- W2044587411 hasVolume "367" @default.
- W2044587411 isParatext "false" @default.
- W2044587411 isRetracted "false" @default.
- W2044587411 magId "2044587411" @default.
- W2044587411 workType "article" @default.