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- W2044606013 abstract "Chromosomal microarray analysis is a growing component of invasive prenatal testing; however, this technique is susceptible to maternal cell contamination (MCC). Many practitioners have abandoned the practice of discarding the first few milliliters (mL) of amniotic fluid withdrawn during the amniocentesis since the reported rate of MCC in cultured cells is considered to be negligible (1-3%). We evaluated the incidence of MCC by molecular analysis within the first 2 mL of amniotic fluid from amniocentesis to determine a more accurate risk of contamination. A prospective observational study was performed between May 2013 and July 2014. The initial 2ml of amniotic fluid withdrawn during the amniocentesis was divided into cultured and uncultured samples for research analysis. A matching maternal buccal swab was obtained for MCC testing. Maternal and fetal DNA was isolated and amplified by PCR. MCC was determined by short tandem repeat analysis. Primary outcomes included the presence or absence of MCC. Additional data collection included gestational age, needle size, location of placenta, volume of amniotic fluid removed, and color of amniotic fluid. Twenty-four samples were acquired for the following indications: advanced maternal age (25%), fetal anomaly (21%), abnormal serum screening (13%), and other indications. Thirteen samples were obtained between 15-21 weeks and eleven > 24 weeks. Three samples did not yield an adequate amount of DNA and 4 failed culture, leaving 17 samples for analysis. Of these samples, 10 uncultured (58.8%) and 2 cultured (11.7%) samples had MCC. Both cultured samples with MCC were obtained from pregnancies with a posterior placenta. Cultured amniotic fluid samples revealed a greater incidence of MCC than previously reported (11.7% vs. 3%). Our data suggests that the practice of discarding the initial 2mL of amniotic fluid from amniocentesis to reduce the risk of maternal cell contamination should not be abandoned." @default.
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- W2044606013 date "2015-01-01" @default.
- W2044606013 modified "2023-10-18" @default.
- W2044606013 title "330: Prevalence of maternal cell contamination in amniotic fluid samples" @default.
- W2044606013 doi "https://doi.org/10.1016/j.ajog.2014.10.376" @default.
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